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Enzyme immunoassays for the demonstration of antibodies to HIV-2SBL-6669 and HTLV-IV (SIVmac).
National bacteriological laboratory.
National bacteriological laboratory.
National bacteriological laboratory.
National bacteriological laboratory.
Vise andre og tillknytning
1988 (engelsk)Inngår i: AIDS (London), ISSN 0269-9370, E-ISSN 1473-5571, Vol. 2, nr 3, s. 195-9Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Enzyme-linked immunosorbent assays (ELISA) were developed for the demonstration of antibodies to HIV-2 using disrupted virions of the SBL-6669 isolate of HIV-2 and the so-called human T-lymphotropic virus type IV (HTLV-IV), recently found to be identical with the simian immunodeficiency virus (SIVmac), as antigens. Three hundred sera from West African subjects, attending an outward clinic in Bissau for examination of suspected tuberculosis, were tested by these two assays as well as by a commercially available anti-HIV-2 ELISA (ELAVIA II). Fifty of these sera were positive in all three ELISAs as well as in Western blot tests against HTLV-IV. Thirty-eight of these positive sera were also tested by an anti-HIV-2 Western blot kit (LAV-Blot II) with positive results. The ELISAs based on SBL-6669 and HTLV-IV antigens had a specificity of 99.6% (one false positive among 250 negative sera) whereas the specificity of ELAVIA II was 94.6% using the recommended cut-off value and 98.4% using a higher cut-off value. Another 58 sera from West African patients, clinically suspected of having AIDS or HIV-related disease, were tested for HIV-2/HTLV-IV antibodies by Western blot and by ELISA against SBL-6669 and HTLV-IV antigens; all of the 30 sera which were positive by Western blot were found to be positive in both ELISAs.(ABSTRACT TRUNCATED AT 250 WORDS)

sted, utgiver, år, opplag, sider
1988. Vol. 2, nr 3, s. 195-9
HSV kategori
Identifikatorer
URN: urn:nbn:se:lnu:diva-21683PubMedID: 3134913OAI: oai:DiVA.org:lnu-21683DiVA, id: diva2:552688
Tilgjengelig fra: 2012-09-14 Laget: 2012-09-14 Sist oppdatert: 2017-12-07bibliografisk kontrollert

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