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Photoinitiated Coupling of Unmodified Monosaccharides to Iron Oxide Nanoparticles for Sensing Proteins and Bacteria
Department of Chemistry, Portland State University.ORCID-id: 0000-0003-1121-4007
2009 (engelsk)Inngår i: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 20, nr 7, s. 1349-1355Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We report a versatile approach for the immobilization of unmodified monosaccharides onto iron oxide nanoparticles. Covalent coupling of the carbohydrate onto iron oxide nanoparticle surfaces was accomplished by the CH insertion reaction of photochemically activated phosphate-functionalized perfluorophenylazides (PFPAs), and the resulting glyconanoparticles were characterized by IR, TGA, and TEM. The surface-bound D-mannose showed the recognition ability toward Concanavalin A and Escherichia coli strain ORN178 that possesses mannose-specific receptor sites. Owing to the simplicity and versatility of the technique, together with the magnetic property of iron oxide nanoparticles, the methodology developed in this study serves as a general approach for the preparation of magnetic glyconanoparticles to be used in clinical diagnosis, sensing, and decontamination.

sted, utgiver, år, opplag, sider
2009. Vol. 20, nr 7, s. 1349-1355
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URN: urn:nbn:se:kth:diva-77358DOI: 10.1021/bc900110xISI: 000268137700009OAI: oai:DiVA.org:kth-77358DiVA, id: diva2:492016
Merknad
QC 20120313Tilgjengelig fra: 2012-02-07 Laget: 2012-02-06 Sist oppdatert: 2017-12-08bibliografisk kontrollert

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