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Development of a multifunctional microfluidic system for studies of nerve cell activity during hypoxic and anoxic conditions
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Fysiologi.
2009 (engelsk)Inngår i: International Federation for Medical and Biological Engineering Proceedings; 25, 2009, s. 176-179Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

 

Abstract

—Hemoproteins usually supply cells and tissue

 with oxygen. A new hemoprotein mainly present in nerve cells called Neuroglobin was recently discovered. Enhanced expression of the protein has been shown to reduce hypoxic neural injury but the mechanism behind this function remains unknown. Methods enabling investigation of the protein in single functional neurons need to be developed. Here, we have studied how the electrical signaling capacity of a neuron was affected by hypoxic environments. Preliminary results show a trend of higher noise-level when a neuron is exposed to hypoxic compared to normoxic surroundings, which implies increased ion-channel activity. The setup used today shows shortages such as reduced control over the oxygen content due to leakage. Therefore, a gas-tight, multifunctional microfluidic system is under development which enables us to study influences of Neuroglobin concentrations on neuronal activity during hypoxia and anoxia. For electrophysiological recordings a patch-clamp micro pipette will be molded into the walls of the microfluidic system. A single biological cell is steered towards the pipette and attached there by means of optical tweezers. The Neuroglobin oxygen binding state will be studied using optical spectroscopy and the neuron environment will be manipulated by applying flows of varying oxygen content through the microfluidic system. This system will constitute a powerful tool in the investigation of the Neuroglobin mechanism of action. 

sted, utgiver, år, opplag, sider
2009. s. 176-179
Emneord [en]
Neuroglobin, hypoxia, multifunctional microfluidic system, patch clamp, optical tweezers, optical spectroscopy
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-42401OAI: oai:DiVA.org:umu-42401DiVA, id: diva2:409191
Konferanse
World Congress on Medical Physics and Biomedical Engineering: September 7-12, 2009 Munich, Germany
Tilgjengelig fra: 2011-04-07 Laget: 2011-04-07 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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