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Electrochemical acetylcholine chloride biosensor using an acetylcholine esterase biomimic
University Calcutta, Department Polymer Science and Technology, Biosensor Lab, Calcutta 700009, India.
University Calcutta, Department Polymer Science and Technology, Biosensor Lab, Calcutta 700009, India.
University Calcutta, Department Polymer Science and Technology, Biosensor Lab, Calcutta 700009, India.
Jadavpur University, Department Chemistry Engn, Calcutta, India.
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2008 (Engelska)Ingår i: Analytical Letters, ISSN 0003-2719, E-ISSN 1532-236X, Vol. 41, nr 8, s. 1387-1397Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Pure enzymes are costly and highly sensitive to change in pH, temperature, ionic strength etc. Hence biomimetic or synthetic enzymes could be useful alternatives to such natural proteins. Although the selectivity of a biomimic is somewhat less than that of enzyme, it can be used as a detector element in inexpensive but stable biosensors. An organic compound, 4-[(1E)-ethanehydrazonoyl]benzoic acid, has been designed and synthesized as biomimic for the enzyme acetylcholine esterase. An acetylcholine chloride two-electrode screen-printed sensor was first developed using the immobilized enzyme acetylcholine esterase. The performance of the mimic in the hydrolysis of acetylcholine chloride was then tested with the same transducer by immobilizing the biomimic in place of the enzyme. The response of the sensor constructed using the mimic was comparable to that of the pure acetylcholine esterase enzyme electrode.

Ort, förlag, år, upplaga, sidor
Taylor andamp;amp; Francis , 2008. Vol. 41, nr 8, s. 1387-1397
Nyckelord [en]
acetylcholine chloride; acetylcholine esterase; biomimic; enzyme; screen-printed sensor
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Teknik och teknologier
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URN: urn:nbn:se:liu:diva-65169DOI: 10.1080/00032710802119251ISI: 000257524800008OAI: oai:DiVA.org:liu-65169DiVA, id: diva2:394911
Tillgänglig från: 2011-02-04 Skapad: 2011-02-04 Senast uppdaterad: 2017-12-11

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