Digitala Vetenskapliga Arkivet

Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Effects of short-term food deprivation on orexin-A-induced intestinal bicarbonate secretion in comparison with related secretagogues
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Fysiologi.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Fysiologi.
Inst. of Biomedicine, Div. of Physiology, Oulu Univ., Oulu, Finland.
Inst. of Biomedicine, Div. of Physiology, Oulu Univ., Oulu, Finland.
2010 (engelsk)Inngår i: Acta Physiologica, ISSN 1748-1708, E-ISSN 1748-1716, Vol. 198, nr 3, s. 373-380Artikkel, forskningsoversikt (Fagfellevurdert) Published
Abstract [en]

Studies of gastrointestinal physiology in humans and intact animals are usually conducted after overnight fast. We have compared effects of orexin-A, vasoactive intestinal polypeptide (VIP), melatonin, serotonin, uroguanylin, ghrelin and prostaglandin E2 (PGE2) on duodenal bicarbonate secretion in fed and overnight fasted animals. This review is a summary of our findings. Secretagogues  were administered by intra-arterial infusion or luminally (PGE2) Enterocyte intracellular calcium ([Ca2+]i) signaling was studied by fluorescence imaging. Total RNA was extracted, reverse transcripted to cDNA and expression of orexin receptors measured by quantitative real-time PCR.

Orexin-A stimulates the duodenal secretion in continuously fed animals but not in food deprived animals. Similarly, short fasting causes a 100-fold decrease  of the amount of the muscarinic agonist bethanechol required for stimulation of secretion. In contrast, fasting does not affect secretory responses to intra-arterial VIP, melatonin, serotonin, uroguanylin and ghrelin, or that to luminal PGE2. Orexin-A induces [Ca2+]i signaling in enterocytes from fed rats but no significant [Ca2+]i responses occurs in enterocytes from fasted animals. In addition, overnight fasting decreases the expression of mucosal and enterocyte orexin receptors.

Short food deprivation thus decreases duodenal expression of orexin receptors and abolishes the secretory response to orexin-A as well as orexin-A induced [Ca2+]i signaling. Fasting, furthermore, decreases mucosal sensitivity to bethanechol. The absence of declines in secretory responses to other secretagogues tested is strong evidence that short fasting does affect not the secretory capacity of the duodenal mucosa in general. Studies of intestinal secretion require particular evaluation with respect to feeding status.

sted, utgiver, år, opplag, sider
Oxford: Wiley-Blackwell , 2010. Vol. 198, nr 3, s. 373-380
Emneord [en]
Bicarbonate secretion, duodenum, fasting, ghrelin, melatonin, serotonin
HSV kategori
Forskningsprogram
Neurovetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-130658DOI: 10.1111/j.1748-1716.2009.02067.xISI: 000274147900019PubMedID: 20003099OAI: oai:DiVA.org:uu-130658DiVA, id: diva2:350210
Prosjekter
Feeding dependency of small intestinal sensitivity to appetite-related hormonesTilgjengelig fra: 2010-09-10 Laget: 2010-09-10 Sist oppdatert: 2017-12-12bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed
Av organisasjonen
I samme tidsskrift
Acta Physiologica

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 432 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf