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FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis.
KTH, Tidigare Institutioner                               , Bioteknologi.ORCID-id: 0000-0003-0996-1644
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1995 (Engelska)Ingår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 92, nr 1, s. 195-9Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

PR-39, a proline/arginine-rich peptide antibiotic, has been purified from pig intestine and later shown to originate in the bone marrow. Intending to isolate a clone for a human counterpart to PR-39, we synthesized a PCR probe derived from the PR-39 gene. However, when this probe was used to screen a human bone marrow cDNA library, eight clones were obtained with information for another putative human peptide antibiotic, designated FALL-39 after the first four residues. FALL-39 is a 39-residue peptide lacking cysteine and tryptophan. All human peptide antibiotics previously isolated (or predicted) belong to the defensin family and contain three disulfide bridges. The clone for prepro-FALL-39 encodes a cathelin-like precursor protein with 170 amino acid residues. We have postulated a dibasic processing site for the mature FALL-39 and chemically synthesized the putative peptide. In basal medium E, synthetic FALL-39 was highly active against Escherichia coli and Bacillus megaterium. Residues 13-34 in FALL-39 can be predicted to form a perfect amphiphatic helix, and CD spectra showed that medium E induced 30% helix formation in FALL-39. RNA blot analyses disclosed that the gene for FALL-39 is expressed mainly in human bone marrow and testis.

Ort, förlag, år, upplaga, sidor
1995. Vol. 92, nr 1, s. 195-9
Nationell ämneskategori
Industriell bioteknik
Identifikatorer
URN: urn:nbn:se:kth:diva-13359PubMedID: 7529412OAI: oai:DiVA.org:kth-13359DiVA, id: diva2:324519
Anmärkning
QC 20100615Tillgänglig från: 2010-06-15 Skapad: 2010-06-15 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
Ingår i avhandling
1. Analysis of human gene transcripts: Identification and characterisation based on homology and differential expression
Öppna denna publikation i ny flik eller fönster >>Analysis of human gene transcripts: Identification and characterisation based on homology and differential expression
1998 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Ort, förlag, år, upplaga, sidor
Stockholm: KTH, 1998. s. 68
Nationell ämneskategori
Industriell bioteknik
Identifikatorer
urn:nbn:se:kth:diva-2751 (URN)91-7170-347-0 (ISBN)
Disputation
1998-12-18, 00:00
Anmärkning
QC 20100615Tillgänglig från: 2000-01-01 Skapad: 2000-01-01 Senast uppdaterad: 2010-06-17Bibliografiskt granskad

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Odeberg, Jacob
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