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Receptors for thyroid-stimulating hormone and thyroid hormones in human ovarian tissue
Dept of Clinical Science, Intervention and Technology, Div of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Dept of Clinical Science, Intervention and Technology, Div of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Dept of Clinical Science, Intervention and Technology, Div of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa. (Klinisk och experimentell reproduktionsbiologi/Olovsson)
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2009 (Engelska)Ingår i: Reproductive Biomedicine Online, ISSN 1472-6483, E-ISSN 1472-6491, Vol. 18, nr 3, s. 337-347Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Dysfunction in thyroid regulation can cause menstrual and ovulatory disturbances, the mechanism of which is not clear. The distribution and activity of the thyroid-stimulating hormone (TSHR), and the thyroid hormone receptors (TR) alpha1, alpha2 and beta1 in human ovarian tissue and in granulosa cells was studied using immunohistochemistry, reverse-transcriptase polymerase chain reaction (RT-PCR), quantitative PCR and immunoassays. Strong immunostaining of TSHR, TRalpha1 and TRbeta1 was observed in ovarian surface epithelium and in oocytes of primordial, primary and secondary follicles, with minimal staining in granulosa cells of secondary follicles. Granulosa cells of antral follicles expressed TSHR, TRalpha1 and TRbeta1 proteins. Messenger RNA for all receptors was present in ovarian tissue. Mature human granulosa cells expressed transcripts for 5' deiodinases types 2 and 3, but not type 1, indicating the possibility of conversion of peripheral thyroid hormone thyroxin (T(4)). Granulosa cells stimulated with TSH showed a significant increase in cAMP concentrations after 2 h of culture (P = 0.047), indicating activation through TSHR. Stimulation with T(4) resulted in increased extracellular signal-regulated kinase 1 and 2 activation after 10, 30, 60 min and 24 h. These data demonstrate that TSH and thyroid hormone receptors may participate in the regulation of ovarian function.

Ort, förlag, år, upplaga, sidor
2009. Vol. 18, nr 3, s. 337-347
Nyckelord [en]
cAMP, ERK1/2, granulosa cells, human ovary, thyroid hormone receptor, thyroid stimulating hormone receptor
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-107818ISI: 000264455200005PubMedID: 19298732OAI: oai:DiVA.org:uu-107818DiVA, id: diva2:233243
Tillgänglig från: 2009-08-31 Skapad: 2009-08-31 Senast uppdaterad: 2017-12-13Bibliografiskt granskad

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