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Morphometrical Methodology in Quantification of Biological Tissue Components
Uppsala universitet, Medicinska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
2004 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Objective:

To develop and validate computer-assisted morphometrical methods, based on stereological theory, in order to facilitate the analysis and quantitative measurements of biological tissue components.

Material and methods:

Biopsy specimens from the vaginal wall or from the vestibulum vaginae of healthy women, or from women suffering from incontinence or vestibulitis were used.

A number of histochemical methods for light microscopy were used, and modified for the different morphometrical analyses. Electron microscopy was used to reveal collagen fibre diameter.

Computer-assisted morphometry, based on image analysis and stereology, was employed to analyse the different tissue components in the biopsies. Computer programs for these purposes were developed and validated.

Results:

The results show that computer-assisted morphometry is of great value for quantitative measurements of the following tissue components:

Epithelium: The epithelial structure, instead of just thickness, was measured in an unbiased way.

Collagen: The collagen fibril diameter was determined in electron microscopic specimens, and the collagen content was analysed in light microscopic specimens.

Elastic fibres: The amount of elastic fibres in the connective tissue was measured after visualisation by autofluorescence.

Vasculature: A stereological method using a cycloid grid was implemented in a computer program. Healthy subjects were compared with patients suffering from vestibulitis. The results were identical in the two groups.

Smooth muscle: A stereological method using a point grid was implemented in a computer program. Using the Delesse principle, the fibres were calculated as area fractions. The area fractions were highly variable among the different specimens.

Conclusion:

Morphometry, used correctly, is an important analysis method in histopathological research. It is important that the methods are as simple and user-friendly as possible. The present studies show that this methodology can be applied for most quantitative histological analyses.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis , 2004. , s. 63
Serie
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1382
Nyckelord [en]
Bildanalys, Morphometry, Stereology, Image analysis, histology
Nyckelord [sv]
Bildanalys
Nationell ämneskategori
Datorseende och robotik (autonoma system)
Identifikatorer
URN: urn:nbn:se:uu:diva-4628ISBN: 91-554-6068-2 (tryckt)OAI: oai:DiVA.org:uu-4628DiVA, id: diva2:165297
Disputation
2004-11-19, Rosénsalen, Akademiska sjukhuset, Uppsala, 09:15
Opponent
Handledare
Tillgänglig från: 2004-10-28 Skapad: 2004-10-28 Senast uppdaterad: 2018-01-13Bibliografiskt granskad
Delarbeten
1. Different organization of collagen fibrils in stress-incontinent women of fertile age
Öppna denna publikation i ny flik eller fönster >>Different organization of collagen fibrils in stress-incontinent women of fertile age
Visa övriga...
1998 Ingår i: Acta Obstetrica et Gynecologica Scandinavica, ISSN 0001-6349, Vol. 77, s. 87-94Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:uu:diva-92277 (URN)
Tillgänglig från: 2004-10-28 Skapad: 2004-10-28Bibliografiskt granskad
2. A computerised stereological method for quantitative estimation of surface area of blood vessels
Öppna denna publikation i ny flik eller fönster >>A computerised stereological method for quantitative estimation of surface area of blood vessels
2001 Ingår i: Image Analysis and Stereology, ISSN 1560-3139, Vol. 20, s. 129-132Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:uu:diva-92278 (URN)
Tillgänglig från: 2004-10-28 Skapad: 2004-10-28Bibliografiskt granskad
3. A novel method for visualisation of elastic fibres - suitable for image analysis and morphometry
Öppna denna publikation i ny flik eller fönster >>A novel method for visualisation of elastic fibres - suitable for image analysis and morphometry
Visa övriga...
2001 Ingår i: Image analysis and stereology, ISSN 1560-3139, Vol. 20, s. 522-526Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:uu:diva-92279 (URN)
Tillgänglig från: 2004-10-28 Skapad: 2004-10-28Bibliografiskt granskad
4. A computerised, unbiased method for epithelial measurement
Öppna denna publikation i ny flik eller fönster >>A computerised, unbiased method for epithelial measurement
Visa övriga...
2004 Ingår i: Micron, Vol. 35, s. 319-329Artikel i tidskrift (Refereegranskat) Published
Identifikatorer
urn:nbn:se:uu:diva-92280 (URN)
Tillgänglig från: 2004-10-28 Skapad: 2004-10-28Bibliografiskt granskad
5. The structure of the normal vaginal wall as revealed by morphometry
Öppna denna publikation i ny flik eller fönster >>The structure of the normal vaginal wall as revealed by morphometry
Artikel i tidskrift (Refereegranskat) Submitted
Identifikatorer
urn:nbn:se:uu:diva-92281 (URN)
Tillgänglig från: 2004-10-28 Skapad: 2004-10-28Bibliografiskt granskad

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