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Bone marrow- and adipose tissue-derived mesenchymal stem cells from donors with coronary artery disease: growth, yield, gene expression and the effect of oxygen concentration
Örebro universitet, Institutionen för medicinska vetenskaper. Department of Laboratory Medicine.ORCID-id: 0000-0002-7954-0696
Örebro universitet, Institutionen för medicinska vetenskaper. Department of Laboratory Medicine.ORCID-id: 0000-0003-2317-5738
Department of Cardiothoracic Surgery, Faculty of Health, Örebro University, Örebro, Sweden.
Region Örebro län. Örebro universitet, Institutionen för medicinska vetenskaper. Department of Cardiothoracic Surgery.ORCID-id: 0000-0003-4249-8401
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2020 (Engelska)Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 80, nr 4, s. 318-326Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Mesenchymal stem cells (MSCs) for cardiovascular cell therapy are procured from different sources including bone marrow and adipose tissue. Differently located MSCs differ in growth potential, differentiation ability and gene expression when cultured in vitro, and studies show different healing abilities for different MSC subgroups. In this study, bone marrow derived MSCs (BMSCs) and adipose tissue derived MSCs (ADSCs) from six human donors with coronary artery disease were compared for growth potential and expression of target genes (Angpt1, LIF, HGF, TGF-β1 and VEGF-A) in response to exposure to 1% and 5% O2, for up to 48 h. We found greater growth of ADSCs compared to BMSCs. ADSCs expressed higher levels of Angpt1, LIF and TGF-β1 and equal levels of VEGF-A and HGF as BMSCs. In BMSCs, exposure to low oxygen resulted in upregulation of TGF-β1, whereas other target genes were unaffected. Upregulation was only present at 1% O2. In ADSCs, LIF was upregulated in both oxygen concentrations, whereas Angpt1 was upregulated only at 1% O2. Different response to reduced oxygen culture conditions is of relevance when expanding cells in vitro prior to administration. These findings indicate ADSCs as better suited for cardiovascular cell therapy compared to BMSCs.

Ort, förlag, år, upplaga, sidor
Taylor & Francis, 2020. Vol. 80, nr 4, s. 318-326
Nyckelord [en]
Adipose tissue, bone marrow, cardiovascular diseases, cell- and tissue-based therapy, heart failure, humans, hypoxia, mesenchymal stem cells
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:oru:diva-80807DOI: 10.1080/00365513.2020.1741023ISI: 000524023000001PubMedID: 32189529Scopus ID: 2-s2.0-85082336592OAI: oai:DiVA.org:oru-80807DiVA, id: diva2:1416400
Tillgänglig från: 2020-03-23 Skapad: 2020-03-23 Senast uppdaterad: 2024-01-16Bibliografiskt granskad

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Adolfsson, EmmaHelenius, GiselaSamano, NinosFröbert, OleJohansson, Karin
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Institutionen för medicinska vetenskaperRegion Örebro län
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Scandinavian Journal of Clinical and Laboratory Investigation
Cell- och molekylärbiologi

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