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TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells
Karolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden..
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science.
King Abdullah Univ Sci & Technol, Comp Elect & Math Sci & Engn Div, Biol & Environm Sci & Engn Div, Thuwal, Saudi Arabia..
Karolinska Inst, Dept Oncol & Pathol, Sci Life Lab, Solna, Sweden..
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2019 (English)In: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 10, article id 2708Article in journal (Refereed) Published
Abstract [en]

We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2019. Vol. 10, article id 2708
Keywords [en]
subcellular fractionation, subcellular localization, CD4 T cells, TCR stimulation, protein translocation, mass spectrometry-based proteomics
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-266307DOI: 10.3389/fimmu.2019.02708ISI: 000501784000001PubMedID: 31849937Scopus ID: 2-s2.0-85076702523OAI: oai:DiVA.org:kth-266307DiVA, id: diva2:1383057
Note

QC 20200107

Available from: 2020-01-07 Created: 2020-01-07 Last updated: 2020-01-07Bibliographically approved

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