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Photosystem ratio imbalance promotes direct sustainable H-2 production in Chlamydomonas reinhardtii
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. John Innes Ctr, Dept Mol Microbiol, Norwich NR4 7UH, Norfolk, England.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.ORCID iD: 0000-0002-6218-3039
2019 (English)In: Green Chemistry, ISSN 1463-9262, E-ISSN 1463-9270, Vol. 21, no 17, p. 4683-4690Article in journal (Refereed) Published
Abstract [en]

The green alga Chlamydomonas reinhardtii can photoproduce H-2 gas for only a few minutes under anaerobic conditions due to the inhibition of hydrogenase by O-2 produced by Photosystem II (PSII). A few days of sustained H-2 production can only be achieved when O-2 and H-2 production are temporally separated under two-stage processes such as sulfur deprivation. Under sulfur deprivation, H-2 production is initiated after the over-reduction of the plastoquinone pool and decreased PSII activity in the thylakoid membrane. As a result, activated hydrogenase consumes the excess of electrons produced by PSII [Volgusheva et al., Proc. Natl. Acad. Sci. U. S. A., 2013, 110, 7223]. Here, we report that similar conditions can be achieved by simply altering the ratio between photosystem I (PSI) and PSII. In the C3 mutant of C. reinhardtii, we found a lower PSI/PSII ratio than in the wild type, 0.33 vs. 0.85, respectively. This imbalance of photosystems resulted in the over-reduced state of the plastoquinone pool and activation of hydrogenase in the C3 mutant that allowed the photoproduction of H-2 continuously for 42 days. This is an unprecedented duration of H-2 production in green algae under standard growth conditions without any nutrient limitation. Photosynthetic electron flow from PSII to hydrogenase was closely regulated during this long-term H-2 production. The amount of PSII was decreased and the amount of PSI was increased reaching a PSI/PSII ratio of more than 5 as shown by EPR and fluorescence spectroscopy. This fine-tuning of photosystems allows to sustain the long-term production of H-2 in C. reinhardtii by a direct photosynthetic pathway.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY , 2019. Vol. 21, no 17, p. 4683-4690
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-394196DOI: 10.1039/c9gc01416kISI: 000483588100011OAI: oai:DiVA.org:uu-394196DiVA, id: diva2:1359184
Funder
Carl Tryggers foundation , CTS14:314Carl Tryggers foundation , CTS15:319Available from: 2019-10-08 Created: 2019-10-08 Last updated: 2019-10-08Bibliographically approved

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