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Casein-Conjugated Gold Nanoparticles for Amperometric Detection of Leishmania infantum
Inst Pasteur Tunis, LR11IPT03, LEMV, Tunis 1002, Tunisia;Univ Tunis El Manar, Tunis 1068, Tunisia.
Prince Sattam Bin Abdulaziz Univ, Al Kahrj 11942, Saudi Arabia;Univ Tunis El Manar, Tunis 1068, Tunisia.ORCID iD: 0000-0001-5520-1799
Inst Pasteur Tunis, LR11IPT03, LEMV, Tunis 1002, Tunisia.
Inst Pasteur Tunis, LR11IPT03, LEMV, Tunis 1002, Tunisia;Univ Tunis El Manar, Tunis 1068, Tunisia.ORCID iD: 0000-0003-1419-6414
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2019 (English)In: Biosensors, ISSN 2079-6374, Vol. 9, no 2, article id 68Article in journal (Refereed) Published
Abstract [en]

Sensitive and reliable approaches targeting the detection of Leishmania are critical for effective early diagnosis and treatment of leishmaniasis. In this frame, this paper describes a rapid quantification assay to detect Leishmania parasites based on the combination of the electrocatalytic ability of gold nanoparticles (AuNPs) to act as a catalyst for the hydrogen formation reaction along with the specificity of the interaction between casein and the major surface protease of the Leishmania parasite, GP63. First, pure and casein-modified AuNPs were prepared and characterized by scanning electron microscopy and ultraviolet-visible spectroscopy. Then, casein-conjugated AuNPs were incubated with Leishsmania parasites in solution; the formed complex was collected by centrifugation, treated by acidic solution, and the pelleted AuNPs were placed on screen-printed carbon electrodes (SPCEs) and chronoamperometric measurements were carried out. Our results suggest that it is possible to detect Leishmania parasites, with a limit less than 1 parasite/mL. A linear response over a wide concentration interval, ranging from 2 x 10(-2) to 2 x 10(5) parasites/mL, was achieved. Additionally, a pretreatment of Leishmania parasites with Amphotericin B, diminished their interaction with casein. This findings and methodology are very useful for drug efficacy assessment.

Place, publisher, year, edition, pages
2019. Vol. 9, no 2, article id 68
Keywords [en]
Leishmania infantum, gold nanoparticles, casein, chronoamperometry, GP63
National Category
Physical Chemistry Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-392053DOI: 10.3390/bios9020068ISI: 000477008100022PubMedID: 31137793OAI: oai:DiVA.org:uu-392053DiVA, id: diva2:1349866
Available from: 2019-09-10 Created: 2019-09-10 Last updated: 2019-09-10Bibliographically approved

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