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Characterization of antibodies against mustard and development of immunological methods for the detection and quantification of mustard in foods
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2007 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

ABSTRACT

Allergy to mustard has been reported for many years, in some cases as severe anaphylactic reactions. Recent studies imply that this allergy is increasing. Three major allergens have been isolated and characterised; Sin a 1 and Sin a 2 in yellow mustard (Sinapis alba), and Bra j 1 in oriental mustard (Brassica juncea). Yellow mustard and black mustard (Brassica nigra) are the most common species in Europe, whereas oriental mustard is more frequent outside Europe. Mustard plants belong to the Brassicaceae/Cruciferae family. Mustard is present as an ingredient in different foods, sauces and spices, often in small amounts. According to the European labelling directives, mustard and products thereof must always be declared. To monitor this regulation, methods need to be developed to detect mustard. Polyclonal antibodies, produced in rabbits, against yellow and black mustard were characterised with immunodiffusion, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions, and immunoblotting. Rocket-immunoelectrophoresis and enzyme-linked immunosorbent assay (ELISA) were developed for the detection and quantification of mustard protein. With indirect competitive ELISA a concentration of 156ng mustard protein per ml food extract was detected, which is more than enough to cover the lowest reported reactive doses.

Place, publisher, year, edition, pages
Uppsala: Universitetsbiblioteket , 2007. , p. 25
Keywords [en]
Mustard allergen; rocket-immunoelectrophoresis; ELISA; immunoblotting; immunodiffusion.
National Category
Immunology
Identifiers
URN: urn:nbn:se:uu:diva-8093OAI: oai:DiVA.org:uu-8093DiVA, id: diva2:131570
Uppsok
bio-/geovetenskap
Supervisors
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Available from: 2007-06-29 Created: 2007-06-29

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Department of Medical Biochemistry and Microbiology
Immunology

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CiteExportLink to record
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