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Generic Neutravidin Biosensor for Simultaneous Multiplex Detection of MicroRNAs via Electrochemically Encoded Responsive Nanolabels
Not Found:Linkoping Univ, Biosensors and Bioelect Ctr, Dept Phys Chem and Biol IFM, S-58183 Linkoping, Sweden; Univ Sousse, Tunisia; Ctr Res Microelect and Nanotechnol Sousse, Tunisia.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska fakulteten. Univ Sousse, Tunisia; Ctr Res Microelect and Nanotechnol Sousse, Tunisia.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensor- och aktuatorsystem. Linköpings universitet, Tekniska fakulteten. Cranfield Univ, England.ORCID-id: 0000-0002-1815-9699
Univ Sousse, Tunisia; Ctr Res Microelect and Nanotechnol Sousse, Tunisia.
Vise andre og tillknytning
2019 (engelsk)Inngår i: ACS SENSORS, ISSN 2379-3694, Vol. 4, nr 2, s. 326-334Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Current electrochemical biosensors for multiple miRNAs require tedious immobilization of various nucleic acid probes. Here, we demonstrate an innovative approach using a generic neutravidin biosensor combined with electrochemically encoded responsive nanolabels for facile and simultaneous multiplexed detection of miRNA-21 and miRNA-141. The selectivity of the biosensor arises from the intrinsic properties of the electrochemically encoded responsive nanolabels, comprising biotinylated molecular beacons (biotin-MB) and metal nanoparticles (metal-NPs). The procedure is a simple one-pot assay, where the targeted miRNA causes the opening of biotin-MB followed by capturing of the biotin-MB-metal-NPs by the neutravidin biosensor and simultaneous detection of the captured metal-NPs by stripping square-wave voltammetry (SSWV). The multiplexed detection of miRNA-21 and miRNA-141 is achieved by differentiation of the electrochemical signature (i.e., the peak current) for the different metal-NP labels. The biosensor delivers simultaneous detection of miRNAs with a linear range of 0.5-1000 pM for miRNA-21 and a limit of detection of 0.3 pM (3 sigma/sensitivity, n = 3), and a range of 50-1000 pM for miRNA-141, with a limit of detection of 10 pM. Furthermore, we demonstrate multiplexed detection of miRNA-21 and miRNA-141 in a spiked serum sample.

sted, utgiver, år, opplag, sider
AMER CHEMICAL SOC , 2019. Vol. 4, nr 2, s. 326-334
Emneord [en]
neutravidin biosensor; multiplex detection; microRNAs; molecular beacons; nanoparticle labels
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Identifikatorer
URN: urn:nbn:se:liu:diva-155593DOI: 10.1021/acssensors.8b00942ISI: 000459836400009PubMedID: 30730699OAI: oai:DiVA.org:liu-155593DiVA, id: diva2:1297817
Merknad

Funding Agencies|EU-FP7 project [PIRSES-GA-2012-318053]

Tilgjengelig fra: 2019-03-21 Laget: 2019-03-21 Sist oppdatert: 2019-09-11

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