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Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Marine Microbial Ecology)
2018 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Bacterioplankton provide important ecosystem functions by carrying out biogeochemical cycling of organic matter. Playing an important role in the microbial loop they help remineralize carbon and nutrients. Bacteria also interact with phytoplankton during phytoplankton blooms. However, fundamental understanding on the underlying molecular mechanisms involved in the degradation of phytoplankton-derived organic matter is still in its infancy. Therefore, we analysed data from a mesocosm experiment following a natural phytoplankton-bloom from an upwelling system in the North- East Atlantic Ocean. The purpose was to contribute a mechanistic understanding based on functional gene expression analysis of natural microbial assemblages. Our results show the difference in functional gene expression within a bacterial metacommunity and how this functional response drastically switches between bloom build up and senescence. Transcripts showed a broad change in gene expression involving major SEED categories, with the bloom senescence phase exhibiting a higher relative abundance in major categories such as Carbohydrates, Protein Metabolism and Amino Acids and Derivatives. Within these categories genes connected to carbon utilization and transport systems (Ton and Tol) as well as chemotaxis showed a higher abundance during bloom senescence. The change in functionality based on transcripts showed a different bacterial community composition appearing over a very short time. We thus conclude that the bacterial functional gene expression response between build-up and degradation bloom phases is remarkably different and associated with a change in the identity of bacteria with active expression. Our findings highlight the importance of bacterial substrate specialists with different functional roles during different time points of phytoplankton blooms.

Place, publisher, year, edition, pages
2018. , p. 27
Keywords [en]
Transcriptomics, bacteria, microbes, DOM, marine environment, bioinformatics
National Category
Other Biological Topics Bioinformatics and Systems Biology
Identifiers
URN: urn:nbn:se:lnu:diva-79200OAI: oai:DiVA.org:lnu-79200DiVA, id: diva2:1270617
Subject / course
Biology
Educational program
Akvatisk ekologi, masterprogram, 120 hp
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Examiners
Available from: 2018-12-14 Created: 2018-12-13 Last updated: 2018-12-14Bibliographically approved

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Master_arbete_DA(2148 kB)60 downloads
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Type fulltextMimetype application/pdf

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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Language
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  • Other locale
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Output format
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