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A fine-needle aspiration-based protein signature discriminates benign from malignant breast lesions
Karolinska Inst, Canc Ctr Karolinska, Dept Pathol & Oncol, Stockholm, Sweden; Univ Hosp, Stockholm, Sweden.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.ORCID iD: 0000-0002-1303-2218
Karolinska Inst, Dept Microbiol Tumor & Cell Biol MTC, Stockholm, Sweden;Natl Bioinformat Infrastruct Sweden, Sci Life Lab, Solna, Sweden.ORCID iD: 0000-0001-8812-6481
Karolinska Inst, Canc Ctr Karolinska, Dept Pathol & Oncol, Stockholm, Sweden; Univ Hosp, Stockholm, Sweden.
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2018 (English)In: Molecular Oncology, ISSN 1574-7891, E-ISSN 1878-0261, Vol. 12, no 9, p. 1415-1428Article in journal (Refereed) Published
Abstract [en]

There are increasing demands for informative cancer biomarkers, accessible via minimally invasive procedures, both for initial diagnostics and to follow-up personalized cancer therapy. Fine-needle aspiration (FNA) biopsy provides ready access to relevant tissues; however, the minute sample amounts require sensitive multiplex molecular analysis to achieve clinical utility. We have applied proximity extension assays (PEA) and NanoString (NS) technology for analyses of proteins and of RNA, respectively, in FNA samples. Using samples from patients with breast cancer (BC, n=25) or benign lesions (n=33), we demonstrate that these FNA-based molecular analyses (a) can offer high sensitivity and reproducibility, (b) may provide correct diagnosis in shorter time and at a lower cost than current practice, (c) correlate with results from routine analysis (i.e., benchmarking against immunohistochemistry tests for ER, PR, HER2, and Ki67), and (d) may also help identify new markers related to immunotherapy. A specific 11-protein signature, including FGF binding protein 1, decorin, and furin, distinguished all cancer patient samples from all benign lesions in our main cohort and in smaller replication cohort. Due to the minimally traumatic sampling and rich molecular information, this combined proteomics and transcriptomic methodology is promising for diagnostics and evaluation of treatment efficacy in BC.

Place, publisher, year, edition, pages
2018. Vol. 12, no 9, p. 1415-1428
Keywords [en]
breast cancer diagnosis, fine-needle aspiration, protein biomarker, proximity extension assay
National Category
Cancer and Oncology Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-364195DOI: 10.1002/1878-0261.12350ISI: 000443402000002PubMedID: 30019538OAI: oai:DiVA.org:uu-364195DiVA, id: diva2:1259303
Funder
VINNOVA, 201600595Swedish Research CouncilEU, FP7, Seventh Framework Programme, 316929 294409Stockholm County Council, 20151043The Breast Cancer FoundationSwedish Cancer SocietyThe Cancer Research Funds of RadiumhemmetAvailable from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-11-16Bibliographically approved

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Kamali-Moghaddam, MasoodAlexeyenko, AndreyWik, LottaLandegren, Ulf
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