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Screening of novel feruloyl esterases from Talaromyces wortmannii for the development of efficient and sustainable syntheses of feruloyl derivatives
Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Kemiteknik.ORCID-id: 0000-0002-7754-9398
Dupont Industrial Biosciences.
Taros Chemicals GmbH & Co KG.
Taros Chemicals GmbH & Co KG.
Vise andre og tillknytning
2019 (engelsk)Inngår i: Enzyme and microbial technology, ISSN 0141-0229, E-ISSN 1879-0909, Vol. 120, s. 124-135Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The feruloyl esterases Fae125, Fae7262 and Fae68 from Talaromyces wortmannii were screened in 10 different solvent: buffer systems in terms of residual hydrolytic activity and of the ability for the transesterification of vinyl ferulate with prenol or L-arabinose. Among the tested enzymes, the acetyl xylan-related Fae125 belonging to the phylogenetic subfamily 5 showed highest yield and selectivity for both products in alkane: buffer systems (n-hexane or n-octane). Response surface methodology, based on a 5-level and 6-factor central composite design, revealed that the substrate molar ratio and the water content were the most significant variables for the bioconversion yield and selectivity. The effect of agitation, the possibility of DMSO addition and the increase of donor concentration were investigated. After optimization, competitive transesterification yields were obtained for prenyl ferulate (87.5-92.6%) and L-arabinose ferulate (56.2-61.7%) at reduced reaction times (≤ 24 h) resulting in good productivities (> 1 g/L/h, >300 kg product/kg FAE). The enzyme could be recycled for six consecutive cycles retaining 66.6% of the synthetic activity and 100% of the selectivity.

sted, utgiver, år, opplag, sider
Elsevier, 2019. Vol. 120, s. 124-135
HSV kategori
Forskningsprogram
Biokemisk processteknik
Identifikatorer
URN: urn:nbn:se:ltu:diva-70723DOI: 10.1016/j.enzmictec.2018.08.007ISI: 000452576500016PubMedID: 30396393Scopus ID: 2-s2.0-85053620494OAI: oai:DiVA.org:ltu-70723DiVA, id: diva2:1244712
Merknad

Validerad;2018;Nivå 2;2018-11-08 (johcin)

Tilgjengelig fra: 2018-09-03 Laget: 2018-09-03 Sist oppdatert: 2019-02-01bibliografisk kontrollert

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Antonopoulou, IoRova, UlrikaChristakopoulos, Paul
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