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Exploring the Viral Channel Kcv(PBCV-1) Function via Computation
KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-0828-3899
2018 (English)In: Journal of Membrane Biology, ISSN 0022-2631, E-ISSN 1432-1424, Vol. 251, no 3, p. 419-430Article in journal (Refereed) Published
Abstract [en]

Viral potassium channels (Kcv) are homologous to the pore module of complex -selective ion channels of cellular organisms. Due to their relative simplicity, they have attracted interest towards understanding the principles of conduction and channel gating. In this work, we construct a homology model of the open state, which we validate by studying the binding of known blockers and by monitoring ion conduction through the channel. Molecular dynamics simulations of this model reveal that the re-orientation of selectivity filter carbonyl groups coincides with the transport of potassium ions, suggesting a possible mechanism for fast gating. In addition, we show that the voltage sensitivity of this mechanism can originate from the relocation of potassium ions inside the selectivity filter. We also explore the interaction of with the surrounding bilayer and observe the binding of lipids in the area between two adjacent subunits. The model is available to the scientific community to further explore the structure/function relationship of Kcv channels.

Place, publisher, year, edition, pages
SPRINGER , 2018. Vol. 251, no 3, p. 419-430
Keywords [en]
Viral ion channel, Homology modeling, Molecular dynamics simulations, Gating, Conduction, Protein-lipid interaction
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-232416DOI: 10.1007/s00232-018-0022-2ISI: 000437103200012PubMedID: 29476260Scopus ID: 2-s2.0-85044948443OAI: oai:DiVA.org:kth-232416DiVA, id: diva2:1235467
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20180725

Available from: 2018-07-25 Created: 2018-07-25 Last updated: 2018-08-21Bibliographically approved

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Andersson, Alma E. V.Kasimova, Marina A.Delemotte, Lucie
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