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The Effects of Cell Culture Medium and Supplements on the Differentiation of Boundary Cap Neural Crest Stem Cells
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa.
2016 (engelsk)Independent thesis Basic level (professional degree), 10 poäng / 15 hpOppgave
Abstract [en]

Boundary cap neural crest stem cells (bNCSCs) are multipotent cells that form a barrier between CNS and PNS, playing an important role in ingrowth of neurites into the spinal cord during development. Because of the stemness and multipotency of bNCSCs, they self-renew and can be used directly for transplantation or as a source of matured neural cells. It is important that cells used for cell therapy differentiate and develop into the mature cells that the recipient needs. To ensure this, cells are guided towards specific cell fates, and one way of doing this is with medium supplements. The purpose of this study was to analyze the effects of three different media with supplements on the differentiation of bNCSCs. Two cell lineages of bNCSCs expressing green- and red fluorescent protein were treated with different media for differentiation. The effects of the media supplements neurotrophic glial cell line-derived neurotrophic factor (GDNF), cilinary neurotrophic factor (CTNF) and fetal bovine serum (FBS) were compared, with one medium containing no additional factors. It was found that when GDNF and CTNF are supplemented in the differentiation media, bNCSCs are guided towards astrocytes. Interestingly, the medium containing no additional factors gave rise to an even amount of neurons and astrocytes. FBS had an inhibitory effect on overall differentiation of bNCSCs, giving rise to the smallest amount of neurons and astrocytes. The bNCSCs are promising for cell therapy, as their differentiation can be guided with the use of medium supplements.

sted, utgiver, år, opplag, sider
2016.
Emneord [en]
GDNF, CTNF, FBS, neurodegenerative diseases, cell therapy
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-349012OAI: oai:DiVA.org:uu-349012DiVA, id: diva2:1199230
Utdanningsprogram
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiner
Tilgjengelig fra: 2018-04-20 Laget: 2018-04-19 Sist oppdatert: 2018-04-20bibliografisk kontrollert

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