Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H
Stockholm University, Faculty of Science, Department of Organic Chemistry.ORCID iD: 0000-0001-5657-8635
Show others and affiliations
2017 (English)In: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology, ISSN 0003-6072, E-ISSN 1572-9699, Vol. 110, no 11, p. 1377-1387Article in journal (Refereed) Published
Abstract [en]

Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: -> 4)-beta-d-GlcpNAcA-(1 -> 3)-beta-d-QuipNAc4NAc-(1 -> 3)-beta-d-GalpNAc-(1 ->. The 3D model, generated in accordance with H-1,H-1-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 A degrees C, this molecule displays only a low ice recrystallization inhibition activity.

Place, publisher, year, edition, pages
2017. Vol. 110, no 11, p. 1377-1387
Keywords [en]
Cold adaptation, Extracellular polysaccharides, NMR, Psychrophile, Anti-freeze activity
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:su:diva-148959DOI: 10.1007/s10482-017-0834-6ISI: 000413219700002PubMedID: 28161737OAI: oai:DiVA.org:su-148959DiVA, id: diva2:1163818
Conference
7th Biennial Baltic Meeting on Microbial Carbohydrates (BMMC), Gustrow, Germany, September 25-29, 2016
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Note

Open Access 2019-09-01

Available from: 2017-12-08 Created: 2017-12-08 Last updated: 2019-09-06Bibliographically approved

Open Access in DiVA

fulltext(756 kB)6 downloads
File information
File name FULLTEXT01.pdfFile size 756 kBChecksum SHA-512
e19a15df97f5b234e6a73a4e8203189a0cc1a29168eb2ae9f7a29a32f3fc5efbb14caefcbc5a054b9da64623a600a767d8de22c5bbf001cce465b0355fe6985c
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ståhle, JonasWidmalm, GöranTutino, Maria L.
By organisation
Department of Organic Chemistry
In the same journal
Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 6 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 37 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf