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Effects of GABA(B) receptors in the insula on recognition memory observed with intellicage
Ningxia Med Univ, Ningxia Key Lab Cerebrocranial Dis, Incubat Base Natl Key Lab, Ningxia, Peoples R China.;Ningxia Med Univ, Gen Hosp, Dept Neurosurg, Ningxia, Peoples R China..
Ningxia Med Univ, Ningxia Key Lab Cerebrocranial Dis, Incubat Base Natl Key Lab, Ningxia, Peoples R China.;Ningxia Med Univ, Gen Hosp, Dept Neurosurg, Ningxia, Peoples R China..
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för neurovetenskap, Fysiologi.ORCID-id: 0000-0002-4717-1558
Ningxia Med Univ, Ningxia Key Lab Cerebrocranial Dis, Incubat Base Natl Key Lab, Ningxia, Peoples R China.;Ningxia Med Univ, Gen Hosp, Dept Neurosurg, Ningxia, Peoples R China..
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2017 (Engelska)Ingår i: Behavioral and Brain Functions, ISSN 1744-9081, E-ISSN 1744-9081, Vol. 13, nr 7Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Insular function has gradually become a topic of intense study in cognitive research. Recognition memory is a commonly studied type of memory in memory research. GABA(B)R has been shown to be closely related to memory formation. In the present study, we used intellicage, which is a new intelligent behavioural test system, and a bilateral drug microinjection technique to inject into the bilateral insula, to examine the relationship between GABA(B)R and recognition memory. Methods: Male Sprague- Dawley rats were randomly divided into control, Sham, Nacl, baclofen and CGP35348 groups. Different testing procedures were employed using intellicage to detect changes in rat recognition memory. The expression of GABA(B)R (GB1, GB2) in the insula of rats was determined by immunofluorescence and western blotting at the protein level. In addition, the expression of GABA(B)R (GB1, GB2) was detected by RT-PCR at the mRNA level. Results: The results of the intellicage test showed that recognition memory was impaired in terms of position learning, punitive learning and punitive reversal learning by using baclofen and CGP35348. In position reversal learning, no significant differences were found in terms of cognitive memory ability between the control groups and the CGP and baclofen groups. Immunofluorescence data showed GABA(B)R (GB1, GB2) expression in the insula, while data from RTPCR and western blot analysis demonstrated that the relative expression of GB1 and GB2 was significantly increased in the baclofen group compared with the control groups. In the CGP35348 group, the expression of GB1 and GB2 was significantly decreased, but there was no significant difference in GB1 or GB2 expression in the control groups. Conclusions: GABA(B)R expression in the insula plays an important role in the formation of recognition memory in rats.

Ort, förlag, år, upplaga, sidor
2017. Vol. 13, nr 7
Nyckelord [en]
GABA(B)R, Insula, Recognition of memory, Intellicage
Nationell ämneskategori
Neurologi
Identifikatorer
URN: urn:nbn:se:uu:diva-322808DOI: 10.1186/s12993-017-0125-4ISI: 000399710300001PubMedID: 28416021OAI: oai:DiVA.org:uu-322808DiVA, id: diva2:1112350
Tillgänglig från: 2017-06-20 Skapad: 2017-06-20 Senast uppdaterad: 2017-11-29Bibliografiskt granskad

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