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Genital Injury Signatures and Microbiome Alterations Associated With Depot Medroxyprogesterone Acetate Usage and Intravaginal Drying Practices
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0002-4657-8532
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2017 (Engelska)Ingår i: Journal of Infectious Diseases, ISSN 0022-1899, E-ISSN 1537-6613, Vol. 215, nr 4, s. 590-598Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background. Increasing evidence suggests depot medroxyprogesterone acetate (DMPA) and intravaginal practices may be associated with human immunodeficiency virus (HIV-1) infection risk; however, the mechanisms are not fully understood. This study evaluated the effect of DMPA and intravaginal practices on the genital proteome and microbiome to gain mechanistic insights. Methods. Cervicovaginal secretions from 86 Kenyan women, including self-reported DMPA users (n = 23), nonhormonal contraceptive users (n = 63), and women who practice vaginal drying (n = 46), were analyzed using tandem-mass spectrometry. Results. We identified 473 human and 486 bacterial proteins from 18 different genera. Depot medroxyprogesterone acetate use associated with increased hemoglobin and immune activation (HBD, HBB, IL36G), and decreased epithelial repair proteins (TFF3, F11R). Vaginal drying associated with increased hemoglobin and decreased phagocytosis factors (AZU1, MYH9, PLAUR). Injury signatures were exacerbated in DMPA users who also practiced vaginal drying. More diverse (H index: 0.71 vs 0.45; P =.009) bacterial communities containing Gardnerella vaginalis associated with vaginal drying, whereas DMPA showed no significant association with community composition or diversity. Conclusions. These findings provide new insights into the impact of DMPA and vaginal drying on mucosal barriers. Future investigations are needed to confirm their relationship with HIV risk in women.

Ort, förlag, år, upplaga, sidor
Oxford University Press, 2017. Vol. 215, nr 4, s. 590-598
Nyckelord [en]
Mucosa, proteomics, DMPA, Depot medroxyprogesterone acetate, microbiome, metaproteome, HIV, progestin, vaginal drying, genital injury
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Identifikatorer
URN: urn:nbn:se:kth:diva-205522DOI: 10.1093/infdis/jiw590ISI: 000398422100014Scopus ID: 2-s2.0-85017032135OAI: oai:DiVA.org:kth-205522DiVA, id: diva2:1094226
Forskningsfinansiär
VetenskapsrådetScience for Life Laboratory - a national resource center for high-throughput molecular bioscienceVINNOVAKnut och Alice Wallenbergs Stiftelse
Anmärkning

QC 20170509

Tillgänglig från: 2017-05-09 Skapad: 2017-05-09 Senast uppdaterad: 2018-01-13Bibliografiskt granskad

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Nilsson, Peter
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Proteomik och nanobioteknologiScience for Life Laboratory, SciLifeLab
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Journal of Infectious Diseases
Mikrobiologi inom det medicinska området

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