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Aspen phenylpropanoid genes’ expression levels correlate with genets’ tannin richness and vary both in responses to soil nitrogen and associations with phenolic profiles
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC). Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. (Benedicte Albrectsen)ORCID-id: 0000-0001-8007-2685
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
Vise andre og tillknytning
2017 (engelsk)Inngår i: Tree Physiology, ISSN 0829-318X, E-ISSN 1758-4469, Vol. 37, nr 2, s. 270-279Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Condensed tannin (CT) contents of European aspen (Populus tremula L.) vary among genotypes, and increases in nitrogen (N) availability generally reduce plants’ tannin production in favor of growth, through poorly understood mechanisms. We hypothesized that intrinsic tannin production rates may co-vary with gene expression responses to soil N and resource allocation within the phenylpropanoid pathway (PPP). Thus, we examined correlations between soil N levels and both expression patterns of eight PPP genes (measured by quantitative-reverse transcription PCR) and foliar phenolic compounds (measured by liquid chromatography–mass spectrometry) in young aspen genets with intrinsically extreme CT levels. Monitored phenolics included salicinoids, lignins, flavones, flavonols, CT precursors and CTs. The PPP genes were consistently expressed more strongly in high-CT trees. Low N supplements reduced expression of genes throughout the PPP in all genets, while high N doses restored expression of genes at the beginning and end of the pathway. These PPP changes were not reflected in pools of tannin precursors, but varying correlations between gene expression and foliar phenolic pools were detected in young and mature leaves, suggesting that processes linking gene expression and the resulting phenolics vary spatially and temporally. Precursor fluxes suggested that CT-related metabolic rate or sink controls are linked to intrinsic carbon allocation strategies associated with N responses. Overall, we found more negative correlations (indicative of allocation trade-offs) between PPP gene expression and phenolic products following N additions in low-CT plants than in high-CT plants. The tannin-related expression dynamics suggest that, in addition to defense, relative tannin levels may also be indicative of intraspecific variations in the way aspen genets respond to soil fertility. 

sted, utgiver, år, opplag, sider
2017. Vol. 37, nr 2, s. 270-279
Emneord [en]
condensed tannins, European aspen, genotypic variation, nitrogen addition, phenylpropanoid pathway, Populus tremula, salicinoids
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-126704DOI: 10.1093/treephys/tpw118ISI: 000397052300012OAI: oai:DiVA.org:umu-126704DiVA, id: diva2:1034878
Tilgjengelig fra: 2016-10-13 Laget: 2016-10-13 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Inngår i avhandling
1. Phenolics, Nitrogen, and Biotic Interactions: A Study of Phenylpropanoid Metabolites and Gene Expression in the Leaves of Populus tremula.
Åpne denne publikasjonen i ny fane eller vindu >>Phenolics, Nitrogen, and Biotic Interactions: A Study of Phenylpropanoid Metabolites and Gene Expression in the Leaves of Populus tremula.
2016 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

European aspen (Populus tremula) is a fast growing tree species, rich in phenolic compounds. Defense theories suggest that soil nitrogen greatly influence plant allocation togrowth and defense; however, the allocation priorities are not well understood. Further,although foliar phenolic compounds are considered defensive, specialist organisms may positively associate with and alter them. There are two classes of phenolics in aspen,condensed tannins (CTs) and salicinoids. They are likely to shape the interactions of themany organisms, for example, herbivorous insects and endophytic fungi and three-way interactions among host genotype, specialist herbivores and endophytic fungi could be greatly altered by aspen geno- and chemotypes’ responses to soil nitrogen.

Firstly, I focused on the allocation of carbon to growth and defense in aspen genotypes with varied tannin content in response to nutrient addition. Nitrogen promoted plant growth and suppressed foliar CT levels. At the molecular level expression of genes of the phenylpropanoid pathway (PPP) decreased under low additions of N (equivalent to 15kg/ha), whereas genes at the beginning and at the end of the pathway increased in response to high levels of N (~150 kg/ha). Aspens high in CTs displayed consistently stronger PPP gene expressions compared to CT-low aspens, and correlations between PPP genes and phenolic products varied with tannin content, as an effect of leaf age, in response to N enrichment, and individually with genotype. More negative correlations (indicative of allocation trade-offs) between PPP gene expressions and phenolic products were found in aspen genets with low tannin levels compared to aspens with inherently high tannin levels.

Secondly, I studied the connection between foliar phenolic compounds and endophytic fungi in the presence and absence of a specialist herbivorous beetle (Chrysomela tremula) and as an effect of soil nitrogen addition. Richness and abundance of fungal endophytes associated with aspen genotypes and phenolic profile, however this specificity disappeared in the presence of the leaf beetles. Herbivory both enhanced endophyte richness andabundance in the leaves and it also increased in response to nitrogen addition.

sted, utgiver, år, opplag, sider
Umeå: Umeå University, 2016. s. 31
Emneord
Populus tremula, condensed tannins, salicinoids, phenolics, genetic variation, soil nitrogen fertilization, herbivory
HSV kategori
Forskningsprogram
biologi
Identifikatorer
urn:nbn:se:umu:diva-126699 (URN)978-91-7601-590-2 (ISBN)
Disputas
2016-11-04, KB3B1, KBC-huset, Umeå University, Umeå, 10:00 (engelsk)
Opponent
Veileder
Forskningsfinansiär
Swedish Foundation for Strategic Research , RBb08-0003
Tilgjengelig fra: 2016-10-14 Laget: 2016-10-13 Sist oppdatert: 2018-06-09bibliografisk kontrollert

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