Digitala Vetenskapliga Arkivet

Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Field deployment of loop-mediated isothermal amplification for centralized mass-screening of asymptomatic malaria in Zanzibar: a pre-elimination setting
Visa övriga samt affilieringar
2015 (Engelska)Ingår i: Malaria Journal, ISSN 1475-2875, E-ISSN 1475-2875, Vol. 14, artikel-id 205Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: Molecular tools for detection of low-density asymptomatic Plasmodium infections are needed in malaria elimination efforts. This study reports results from the hitherto largest implementation of loop-mediated isothermal amplification (LAMP) for centralized mass screening of asymptomatic malaria in Zanzibar.

METHODS: Healthy individuals present and willing to participate in randomly selected households in 60 villages throughout Zanzibar were screened for malaria by rapid diagnostic tests (RDT). In 50 % of the study households, participants were asked to provide 60 μL of finger-prick blood for additional LAMP screening. LAMP was conducted in two centralized laboratories in Zanzibar, by trained technicians with limited or no previous experience of molecular methods. The LAMP assay was performed with Loopamp(TM) MALARIA Pan/Pf Detection Kit (Eiken Chemical Company, Japan). Samples positive for Plasmodium genus (Pan)-LAMP were re-tested using Plasmodium falciparum-specific LAMP kits.

RESULTS: Paired RDT and LAMP samples were available from 3983 individuals. The prevalence of asymptomatic malaria was 0.5 % (CI 95 % 0.1-0.8) and 1.6 % (CI 95 % 1.1-2.2) by RDT and Pan-LAMP, respectively. LAMP detected 3.4 (CI 95 % 2.2-5.2) times more Plasmodium positive samples than RDT. DNA contamination was experienced, but solved by repetitive decontamination of all equipment and reagents.

CONCLUSIONS: LAMP is a simple and sensitive molecular tool, and has potential in active surveillance and mass-screening programmes for detection of low-density asymptomatic malaria in pre-elimination settings. However, in order to deploy LAMP more effectively in field settings, protocols may need to be adapted for processing larger numbers of samples. A higher throughput, affordable closed system would be ideal to avoid contamination.

Ort, förlag, år, upplaga, sidor
2015. Vol. 14, artikel-id 205
Nationell ämneskategori
Infektionsmedicin
Identifikatorer
URN: urn:nbn:se:uu:diva-256149DOI: 10.1186/s12936-015-0731-2ISI: 000354848600001PubMedID: 25982190OAI: oai:DiVA.org:uu-256149DiVA, id: diva2:824682
Forskningsfinansiär
Vetenskapsrådet, 2009-3785, 2013-6594Tillgänglig från: 2015-06-22 Skapad: 2015-06-22 Senast uppdaterad: 2022-01-28Bibliografiskt granskad

Open Access i DiVA

fulltext(498 kB)255 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 498 kBChecksumma SHA-512
1f6cdd987e3a875e9c0f56fbb425fea9840d3064a694c6b6e5f597fdeb9a15fea64b1fdb7129d4ad729e72c222d4881276cf1242767d9e237cbdabe1db61d535
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMed

Sök vidare i DiVA

Av författaren/redaktören
Mårtensson, Andreas
Av organisationen
Centrum för klinisk forskning i Sörmland (CKFD)
I samma tidskrift
Malaria Journal
Infektionsmedicin

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 255 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 470 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf