Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potentialVisa övriga samt affilieringar
2012 (Engelska)Ingår i: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 77, nr 7, s. 1280-1289Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
Glycerol is, to date, the most widely used cryoprotectant to freeze stallion spermatozoa at concentrations between 2% and 5%. Cryoprotectant toxicity has been claimed to be the single most limiting factor for the success of cryopreservation. In order to evaluate the toxic effects of the concentrations of glycerol used in practice, stallion spermatozoa were incubated in Biggers Whitten and Whittingham (BWW) media supplemented with 0%, 0.5%, 1.5%, 2.5%, 3.5%, and 5% glycerol. In two additional experiments, a hyposmotic (75 mOsm/kg) and a hyperosmotic (900 mOsm/kg) control media were included. Sperm parameters evaluated included cell volume, membrane integrity, lipid peroxidation, caspase 3, 7, and 8 activation, mitochondrial membrane potential, and integrity of the cytoskeleton. Glycerol exerted toxicity at concentrations 3.5% and the maximal toxicity was observed at 5%. The actin cytoskeleton was especially sensitive to glycerol presence, inducing rapid F actin depolymerization at concentrations over 1.5%. The sperm membrane and the mitochondria were other structures affected. The toxicity of glycerol is apparently related to osmotic and nonosmotic effects. In view of our results the concentration of glycerol in the freezing media for stallion spermatozoa should not surpass 2.5%.
Ort, förlag, år, upplaga, sidor
Elsevier , 2012. Vol. 77, nr 7, s. 1280-1289
Nyckelord [en]
Stallion, Sperm, Glycerol, Actin cytoskeleton, Toxicity, Cryopreservation
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:liu:diva-76799DOI: 10.1016/j.theriogenology.2011.10.033ISI: 000302108500003OAI: oai:DiVA.org:liu-76799DiVA, id: diva2:516857
Anmärkning
Funding Agencies|Ministerio de Ciencia e Innovacion-FEDER Madrid, Spain|AGL 2010 20758 (GAN)|Inia|RZ2008-00018-00-00|Junta de Extremadura FEDER GR|10010|
2012-04-202012-04-202017-12-07