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Coexistence and expression profiles of two alternative splice variants of the pheromone receptor gene pre-1 in Neurospora crassa
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Evolutionsbiologi.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för ekologi och genetik, Evolutionsbiologi.
2013 (Engelska)Ingår i: Archives of Microbiology, ISSN 0302-8933, E-ISSN 1432-072X, Vol. 195, nr 10-11, s. 773-780Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

In this study, we show that two splice variants of the pheromone receptor gene (pre-1) transcript coexist in vegetative and reproductive tissues of the filamentous ascomycete fungus Neurospora crassa. The two splice variants differ by intron retention of the last intron, which is predicted to result in a premature stop codon and loss of 322 amino acids in the C-terminal cytosolic region of PRE-1. Using quantitative PCR, we show that expression of the variants is influenced by mating type (mat), with a higher proportion of intron-spliced transcripts in a mat A strain and a higher proportion of the intron-retained variant in a mat a strain. The intron-retained PRE-1 variant is predicted to lack 6 ubiquitination sites that may influence receptor function. In conclusion, N. crassa produce two pre-1 splice variants that display different transcription profiles.

Ort, förlag, år, upplaga, sidor
2013. Vol. 195, nr 10-11, s. 773-780
Nyckelord [en]
Alternative splicing, Gene expression, Neurospora, Pheromone receptor gene
Nationell ämneskategori
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:uu:diva-167872DOI: 10.1007/s00203-013-0923-5ISI: 000325816300011OAI: oai:DiVA.org:uu-167872DiVA, id: diva2:488662
Tillgänglig från: 2012-02-02 Skapad: 2012-02-02 Senast uppdaterad: 2017-12-08Bibliografiskt granskad
Ingår i avhandling
1. On the Evolution of Reproductive Systems in Neurospora
Öppna denna publikation i ny flik eller fönster >>On the Evolution of Reproductive Systems in Neurospora
2012 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The aim of this thesis was to study the evolution of reproductive systems and reproductive traits in the fungal genus Neurospora. More specifically, I have investigated the evolutionary forces shaping the genes involved in sexual reproduction, focusing on mating-type (mat) and pheromone receptor (pre) genes. Neurospora contains species exhibiting three different mating systems, i.e., heterothallism (self-incompatibility), homothallism (self-compatibility) and pseudohomothallism (partial self-incompatibility). First, a robust phylogeny of Neurospora was established. The phylogenetic analyses revealed multiple independent transitions in reproductive life style during the evolutionary history of the genus. We argued for a heterothallic ancestor of the genus, although our subsequent ancestral reconstruction analyses favored a homothallic ancestor. To be able to settle the ancestral mating system, we zoomed in on the structural architecture of the mat-locus in four homothallic species of Neurospora, thought to have arisen from independent transitions. Our results led us to suggest two different genetic mechanisms (translocation and unequal crossover) to explain the transitions in mating system from heterothallism to homothallism. We pointed out that the mating-system transitions in Neurospora are unidirectional, and suggested that transposable elements might be driving the transitions. In conclusion, we suggest a heterothallic ancestor for Neurospora, and that at least six transitions to homothallism and two transitions to pseudohomothallism have occurred in its evolutionary history. Further, we used the phylogeny of Neurospora as a framework to test if the evolution of pre-genes (pre-1 and pre-2) in hetero- and homothallic Neurospora is dependent on mating systems and/or even the homothallic clades themselves (i.e., mating-system and/or switch-dependent). The molecular evolution results suggest that pre-1 and pre-2 are overall functional in both homothallic and heterothallic Neurospora. The molecular evolution of pre-1 seems to be independent of mating-system or homothallic clade, and we detected signs for positive selection in the C-terminal tail. For pre-2 we found no support for mating-system dependent evolution, but indications for switch-dependent evolution. In this study we also included expression analyses of both pre- as well as mat-genes, with the prospect to assess functionality and regulation. During this thesis work, we also performed a phylogenetic study were we found that reproductive genes might be more permeable to introgression than other genes, which is in contrast to theoretical expectations. In the last study, we confirmed the co-existence of two alternative splice variants of the pheromone receptor gene pre-1 in Neurospora crassa, and performed expression profiles studies using quantitative RT-PCR. I hope this thesis work will further strengthen Neurospora as a model for research in evolutionary genetics.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2012. s. 41
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 895
Nyckelord
Neurospora, mating type, pheromone receptor, phylogeny, gene expression, heterothallism, homothallism, pseudohomothallism, alternative splicing
Nationell ämneskategori
Naturvetenskap
Forskningsämne
Biologi med inriktning mot evolutionär genetik
Identifikatorer
urn:nbn:se:uu:diva-167875 (URN)978-91-554-8265-7 (ISBN)
Disputation
2012-03-16, Lindahlsalen, EBC, Norbyvägen 14, Uppsala, 09:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2012-02-24 Skapad: 2012-02-02 Senast uppdaterad: 2012-03-01Bibliografiskt granskad

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Strandberg, RebeckaJohannesson, Hanna
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