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Accuracy of TransferRNA Selection in Protein synthesis
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Biologiska sektionen, Institutionen för biologisk grundutbildning. (Måns Eherenberg)
2011 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)Alternativ titel
Accuracy of TransferRNA Selection in Protein synthesis (Engelska)
Abstract [en]

ACCURACY OF TRANSFER RNA SELECTION IN PROTEIN SY

The ribosome is a rapid magnificent molecular machine that plays an important role in proteinsynthesis and it consists of RNA and protein. The 70S bacterial ribosome comprises twosubunits, 30S and 50S. The 30S small subunit of the bacterial ribosome contains a protein calledS12, encoded by the rpsL gene. The function of this S12 protein is to help arrange the mRNAcorrectly to the ribosome and to interact with transfer RNA (tRNA) to initiate translation.Mutations in the rpsL gene generate phenotypes like resistance, dependence or pseudodependenceto the antibiotic streptomycin in bacteria. It is believed that mutations in the rpsLgene can increase the accuracy of tRNA selection in protein synthesis.The ribosome conducts the selection of tRNA in two steps: the initial selection and theproofreading step. During these multiple steps, the ribosome chooses the cognate aminoacyltRNAsin a ternary complex with EF-Tu and GTP and accommodates in the A site of ribosome.Therefore, the accuracy of the ribosome in selection of cognate aminoacyl-tRNA is crucial for the production of functional polypeptide sequences. Here, three different Escherichia coli strains; wild type MG1655, streptomycin restrictive (SmR) strain res222, and a streptomycin pseudo-dependent (SmP) strain w3110 are used, for studying the accuracy of tRNA selection inprotein synthesis. The mutant SmR shows hyper-accurate phenotype, which means, it has lowerpeptide bond formation efficiency and higher accuracy than the wild type. SmP shows pseudodependentto streptomycin phenotype which means it has higher peptide bond formation efficiency in the presence of antibiotic streptomycin. I have estimated the accuracy of tRNA selection in protein synthesis with enzyme kinetics. The kinetics data of these experiments display that mutant streptomycin restrictive is hyper-accurate and lower peptide bond formation efficiency than the wild type. SmP for the near cognate reaction in presence of antibiotic streptomycin has higher peptide bond formation efficiency than the SmP in absence of antibiotic streptomycin. SmP in presence antibiotic streptomycin has lower accuracy than the SmP in absence of antibiotic streptomycin.

Ort, förlag, år, upplaga, sidor
2011. , s. 35
Serie
Uppsala dissertations from the Faculty of Science, ISSN 0346-6485
Nyckelord [en]
RNA, ribosome, Protein
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:uu:diva-162811OAI: oai:DiVA.org:uu-162811DiVA, id: diva2:461717
Utbildningsprogram
Masterprogram i biologi
Presentation
A7:111, Bio medical center, uppsala (Engelska)
Uppsök
bio-/geovetenskap
Handledare
Examinatorer
Tillgänglig från: 2011-12-19 Skapad: 2011-12-05 Senast uppdaterad: 2011-12-19Bibliografiskt granskad

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