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Capillary electrophoresis coupled to biosensor detection
University Verona, Department Agriculture and Ind Biotechnol, I-37134 Verona, Italy; Cranfield University, Institute BioScience and Technology, Cranfield MK43 0AL, Beds, England; .
University Verona, Department Agriculture and Ind Biotechnol, I-37134 Verona, Italy; Cranfield University, Institute BioScience and Technology, Cranfield MK43 0AL, Beds, England; .
University Verona, Department Agriculture and Ind Biotechnol, I-37134 Verona, Italy; Cranfield University, Institute BioScience and Technology, Cranfield MK43 0AL, Beds, England; .
Cranfield University, UK.ORCID-id: 0000-0002-1815-9699
2000 (Engelska)Ingår i: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 892, nr 02-jan, s. 143-153Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The present review highlights some modern aspects of biosensor revelation, a detection method which has already found a large number of applications in healthcare, food industry and environmental analysis. First, the concept of bio-recognition, which is at the heart of biosensor technology, is discussed, with emphasis on host-guest-like recognition mechanisms. This detection device has been successfully coupled, in its first applications, to chromatographic columns, which allow a high resolution of complex mixtures of analytes prior to interaction with the biosensing unit. The properties of the transducing elements, which should generate a signal (e.g., electrochemical, thermal, acoustic, optical) of proper intensity and of relative fast rise, are additionally evaluated and discussed. The review then focuses on potential applications of biosensing units in capillary electrophoresis (CE) devices. CE appears to be an excellent separation methodology to be coupled to biosensor detection, since it is based on miniaturized electrophoretic chambers, fast analysis times, complete automation in sample handling and data treatment and requires extremely small sample volumes. Although only a few applications of CE-based biosensors have been described up to the present, it is anticipated that this hyphenated technique could have a considerable expansion in the coming years, (C) 2000 Elsevier Science B.V. All rights reserved.

Ort, förlag, år, upplaga, sidor
Elsevier Science B.V., Amsterdam. , 2000. Vol. 892, nr 02-jan, s. 143-153
Nyckelord [en]
reviews; capillary electrophoresis-biosensors detection; biosensors; transducers
Nationell ämneskategori
Teknik och teknologier
Identifikatorer
URN: urn:nbn:se:liu:diva-65233ISI: 000089262800011OAI: oai:DiVA.org:liu-65233DiVA, id: diva2:395001
Tillgänglig från: 2011-02-04 Skapad: 2011-02-04 Senast uppdaterad: 2017-12-11

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