Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Attachment Patterns of Human and Avian Influenza Viruses to Trachea and Colon of 26 Bird Species: Support for the Community Concept
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk biokemi och mikrobiologi. (Zoonosis Science Center)
Uppsala universitet, Science for Life Laboratory, SciLifeLab. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för immunologi, genetik och patologi, Klinisk och experimentell patologi.ORCID-id: 0000-0001-5611-1015
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för medicinsk biokemi och mikrobiologi. (Zoonosis Science Center)
Linnaeus Univ, Ctr Ecol & Evolut Microbial Model Syst, Kalmar, Sweden.
Visa övriga samt affilieringar
2019 (Engelska)Ingår i: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 10, artikel-id 815Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Avian influenza A viruses (AIVs) have a broad host range, but are most intimately associated with waterfowl (Anseriformes) and, in the case of the H13 and H16 subtypes, gulls (Charadriiformes). Host associations are multifactorial, but a key factor is the ability of the virus to bind host cell receptors and thereby initiate infection. The current study aims at investigating the tissue attachment pattern of a panel of AIVs, comprising H3N2, H6N1, H12N5, and H16N3, to avian trachea and colon tissue samples obtained from host species of different orders. Virus attachment was not restricted to the bird species or order from which the virus was isolated. Instead, extensive virus attachment was observed to several distantly related avian species. In general, more virus attachment and receptor expression were observed in trachea than in colon samples. Additionally, a human seasonal H3N2 virus was studied. Unlike the studied AIVs, this virus mainly attached to tracheae from Charadriiformes and a very limited set of avian cola. In conclusion, the reported results highlight the importance of AIV attachment to trachea in many avian species. Finally, the importance of chickens and mallards in AIVs dynamics was illustrated by the abundant AIV attachment observed.

Ort, förlag, år, upplaga, sidor
2019. Vol. 10, artikel-id 815
Nyckelord [en]
virus histochemistry, lectin staining, pattern of virus attachment, avian influenza, birds
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:uu:diva-382841DOI: 10.3389/fmicb.2019.00815ISI: 000464963200002PubMedID: 31057520OAI: oai:DiVA.org:uu-382841DiVA, id: diva2:1317235
Forskningsfinansiär
Vetenskapsrådet, 2015-03877Vetenskapsrådet, 2016-02596Knut och Alice Wallenbergs Stiftelse
Anmärkning

De 2 första författarna delar förstaförfattarskapet.

Tillgänglig från: 2019-05-22 Skapad: 2019-05-22 Senast uppdaterad: 2019-10-24Bibliografiskt granskad
Ingår i avhandling
1. Avian Influenza Virus: Deciphering receptor interactions and their role in interspecies transmission
Öppna denna publikation i ny flik eller fönster >>Avian Influenza Virus: Deciphering receptor interactions and their role in interspecies transmission
2019 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Influenza A virus (IAV) annually infects approximately 5–15 % of the human population, causing ~500,000 deaths globally. Novel IAVs have emerged and spread pandemically in the human population, but have over time established endemic circulation with reduced pathogenicity causing seasonal influenza. The natural reservoir of IAVs is wild waterfowl. The past pandemics have been associated with host switch and have partly or entirely originated from birds, or adapted via passage through pigs (postulated IAV mixing vessel). Understanding IAV interspecies transmission mechanisms is essential for pandemic preparedness. Enzootic circulation of avian IAV (AIV) is concentrated to a few waterfowl species, while other bird species seldom are infected. A species barrier preventing IAV interspecies transmission has been suggested. To investigate IAV host range and mixing vessels, histochemistry studies were conducted with tissues from avian species, pigs, and humans. Virus adaptation to new hosts was studied by challenging tufted ducks and chickens with mallard-derived AIVs, together with AIV receptor tropism and glycoproteomic analysis of receptor distribution. Finally, receptor and tissue tropism in ducks was studied systematically for AIV (H1–16). More abundant AIV attachment to human than pig tissues was observed, questioning the pig mixing vessel theory. Attachment patterns of AIVs to bird tissues was generally broad with abundant attachment to trachea. However, among ducks, pronounced attachment was observed to colon of Anas spp., suggesting that intestinal infection might be restricted to Anas spp., whereas other species may be susceptible to respiratory infection. Tufted ducks and chickens could not be infected by intraesophageal inoculation further supporting this hypothesis. Glycan array analysis revealed 3’SLN, 3’STF, and their fucosylated and sulfated analogues as main AIV receptors. Moreover, AIV Neu5Acα2,6 recognition was widespread. Avian respiratory and intestinal tracts glycoproteomic analysis revealed that avian and mammalian receptor structures are much more similar than earlier thought. Furthermore, observed AIV subtype titer variation in challenged tufted ducks and chickens did not correlate with virus receptor tropism. In summary, this thesis suggests that IAV receptor recognition, in particular α2,3 vs. α2,6 sialylated receptor structures, is less important for the IAV interspecies barrier than previously thought.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2019. s. 72
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 1609
Nyckelord
birds, glycobiology, glycovirology, host range, mixing vessel, virus attachment
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Forskningsämne
Medicinsk virologi
Identifikatorer
urn:nbn:se:uu:diva-395407 (URN)978-91-513-0797-8 (ISBN)
Disputation
2019-12-14, Trippelrummet, Navet, SciLifeLab, BMC, Husargatan 3, Uppsala, 09:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2019-11-21 Skapad: 2019-10-24 Senast uppdaterad: 2019-11-21

Open Access i DiVA

fulltext(2853 kB)56 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 2853 kBChecksumma SHA-512
e8dc999ee91f4fba6be34fb2b0cf51bb6ef5ad84daffdc332488695b5802625db1460be84e648912b534a567f58d40b5be49926044b36d23ebc25d1506d2c990
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMed

Sök vidare i DiVA

Av författaren/redaktören
Eriksson, PerLindskog, CeciliaJärhult, Josef D.Lundkvist, ÅkeOlsen, BjörnEllström, Patrik
Av organisationen
Institutionen för medicinsk biokemi och mikrobiologiScience for Life Laboratory, SciLifeLabKlinisk och experimentell patologiInfektionsmedicin
I samma tidskrift
Frontiers in Microbiology
Mikrobiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 56 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 145 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf