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Principles for different modes of multiple-injection CZE
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för analytisk farmaceutisk kemi.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för analytisk farmaceutisk kemi.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för analytisk farmaceutisk kemi.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för analytisk farmaceutisk kemi.
2008 (Engelska)Ingår i: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 29, nr 19, s. 3952-3958Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

This paper introduces four different modes of multiple-injection CZE (MICZE). The validity of these MICZE models was evaluated by the experimental data. Prior to the application of MICZE, the electrophoretic conditions are developed in the single-injection mode by adjusting different experimental parameters such as pH, type and concentration of buffer additives and temperature. Based on the migration time difference (tmig) between the analyte and the internal standard or injection marker, one or more MICZE modes can be employed. The injection marker is added to the sample to compensate for injection-volume fluctuations. The inter-plug distance is regulated by applying an electrical field over the capillary for a short period of time between each injection. After the final injection, the separation is completed by electrophoresis for a time period corresponding to that in the single-injection mode

Ort, förlag, år, upplaga, sidor
Wiley , 2008. Vol. 29, nr 19, s. 3952-3958
Nyckelord [en]
Moxonidine, Multiple-injection, Oxprenolol, Phenylpropanolamine, Salbutamol
Nationell ämneskategori
Läkemedelskemi
Forskningsämne
Analytisk farmaceutisk kemi
Identifikatorer
URN: urn:nbn:se:uu:diva-86740DOI: 10.1002/elps.200800398ISI: 000261049400002OAI: oai:DiVA.org:uu-86740DiVA, id: diva2:127160
Anmärkning
Conference Information: 7th Asia-Pacific International Symposium on Microscale Separations and Analysis Singapore, SINGAPORE, DEC 17-19, 2007 Tillgänglig från: 2008-12-01 Skapad: 2008-12-01 Senast uppdaterad: 2018-01-13Bibliografiskt granskad
Ingår i avhandling
1. Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
Öppna denna publikation i ny flik eller fönster >>Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Different multiple-injection methodologies and the partial filling technique (PFT) have been utilized for separation of pharmaceuticals by capillary elec-trophoresis.

In multiple-injection capillary zone electrophoresis (MICZE), the samples and all standards, used for construction of the calibration curve, are analyzed within a single run. Four different modes of MICZE have been described by means of equations, which were experimentally verified. The developed equations facilitate the transfer from conventional single-injection CZE to one or more of these MICZE-modes, depending on the selectivity between the analyte and the injection marker. The applicability of two of these modes was then demonstrated by quantification of buserelin and salbutamol, re-spectively in commercially available pharmaceutical products. The content of buserelin in an injection solution was determined to 0.94 mg/ml, which only deviated slightly from the declared concentration (1 mg/ml). An alter-native mode of MICZE, offering a higher number of sequential sample injec-tions, was then utilized for single-run determination of salbutamol in 15 tab-lets, with a labelled content of 8 mg. The average content of the tablets was determined to 7.8 mg, with an intra-tablet variation of 3 % or less.

Moreover, UV- and mass-spectrometric detection of enantiomeric amines, resolved by non-aqueous capillary electrophoresis (NACE), was demon-strated. Separation of enantiomeric amines was achieved using the chiral selector (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid, (-)-DIKGA. Introduction of the non-volatile (-)-DIKGA into the mass-spectrometer was avoided by using the PFT, where the capillary is only partially filled with electrolyte containing the chiral selector.

Ort, förlag, år, upplaga, sidor
Uppsala: Acta Universitatis Upsaliensis, 2008. s. 60
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy, ISSN 1651-6192 ; 75
Nyckelord
Capillary Coating, Chiral Separation, Enantiomers, Mass-spectrometric Detection, Method Validation, Multiple-injection Capillary Zone Electrophoresis, Non-aqueous Capillary Electrophoresis, Organic Solvents, Pharmaceutical Analysis, Quantitative Analysis
Identifikatorer
urn:nbn:se:uu:diva-8841 (URN)978-91-554-7211-5 (ISBN)
Disputation
2008-06-05, B41, BMC, Husargatan 3, Uppsala, 13:15
Opponent
Handledare
Tillgänglig från: 2008-05-14 Skapad: 2008-05-14 Senast uppdaterad: 2011-01-28Bibliografiskt granskad

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Amini, AhmadLodén, HenrikPettersson, CurtArvidsson, Torbjörn
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Electrophoresis
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