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The investigation of the biotransformation products formed by Cunninghamella elegans for different classes of drugs by the use of UPLC Q-TOF MS
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för analytisk farmaceutisk kemi.
2015 (engelsk)Independent thesis Advanced level (professional degree), 20 poäng / 30 hpOppgave
Abstract [en]

The fungus Cunninghamella elegans has in many studies shown to have abiotransformation similar to the metabolism of mammals. If the biotransformation isgeneral, it enables the production of metabolites by the fungus and the use asreference material. The purpose of the project were to examine whether themetabolic process of C. elegans is general, with respect to the formation ofglucosides, and can be applied to different classes of drugs. During the project, theanalyses were performed on a UPLC Q-TOF, run in both MSE and MSMS mode. Themobile phase used consisted of MeOH and 0.1 % formic acid in MQ water. Toincrease the concentration of possible glucosides, the samples were subjected to anacidic or alkaline SPE. Glucosides were detected in the fungal incubates of diclofenac,buprenorphine, norbuprenorphine and oxazepam. For diclofenac, besides twodifferent glucosides (diclofenac glucoside and hydroxylated diclofenac glucoside), ahydroxylated metabolite and a hydroxylated metabolite conjugated with sulfate werediscovered. In the samples containing buprenorphine, the phase I metabolitenorbuprenorphine was also encountered. Further, in the fungal incubates ofdexamethasone a defluorinated metabolite was identified, which is a metabolicpathway never before described for C. elegans.ISSN: 1650

sted, utgiver, år, opplag, sider
2015. , s. 28
Serie
UPTEC K, ISSN 1650-8297 ; 14030
Emneord [en]
UPLC Q-TOF MS, Glucosides, Glucuronides, TEMPO reaction, Cunninghamella elegans
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-248510OAI: oai:DiVA.org:uu-248510DiVA, id: diva2:802328
Eksternt samarbeid
Statens Veterinärmedicinska Anstalt
Utdanningsprogram
Master Programme in Chemical Engineering
Veileder
Examiner
Tilgjengelig fra: 2015-06-23 Laget: 2015-03-30 Sist oppdatert: 2015-06-23bibliografisk kontrollert

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The investigation of the biotransformation products formed by Cunninghamella elegans for different classes of drugs by the use of UPLC Q-TOF MS(1705 kB)167 nedlastinger
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