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Amperometric Biosensor for estimation of Glucose-6-phosphate Using Prussian Blue Nanoparticles.
Department of Polymer Science and Technology, University of Calcutta, India.
Department of Polymer Science and Technology, University of Calcutta, India.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan. (Biosensors & Bioelectronics)ORCID-id: 0000-0002-1815-9699
2013 (engelsk)Inngår i: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 439, nr 2, s. 194-200Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Glucose-6-phosphateplays an important role in carbohydrate metabolism of all living organisms.Compared to the conventional analytical methods available for estimation of glucose-6-phosphate,the biosensors having relative simplicity, specificity, low-cost and fastresponse time are a promising alternative. We have reported a glucose-6-phosphatesensor based on screen-printed electrode utilizing Prussian blue nanoparticlesand enzymes, glucose-6-phosphate dehydrogenase and glutathione reductase. The Prussianblue nanoparticles acted as a mediator enhancing the rate of electrochemical responses.The Fourier transforminfrared spectroscopy and energy-dispersiveX-ray spectroscopy study confirmed the formation of Prussian blue, whereas, the atomic forced microscopy revealed that Prussian bluenanoparticles were about 25-30 nm in diameter. To obtainmaximum amperometric response, optimization studies were conducted for pH,enzyme and cofactor loading. The proposed glucose-6-phosphate biosensor showed goodstability, rapid response time and broad linear response in the range of 0.01-1.25mM and detection limit of 6.3 mM. The biosensor also worked well for serum samples and exhibitedexcellent anti-interference ability.

sted, utgiver, år, opplag, sider
2013. Vol. 439, nr 2, s. 194-200
Emneord [en]
Glucose-6-phosphate; Prussian blue nanoparticles; Screen-printed electrode; Amperometry
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Identifikatorer
URN: urn:nbn:se:liu:diva-91855DOI: 10.1016/j.ab.2013.04.025ISI: 000322286900017OAI: oai:DiVA.org:liu-91855DiVA, id: diva2:619344
Tilgjengelig fra: 2013-05-03 Laget: 2013-05-03 Sist oppdatert: 2017-12-06

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