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YopH of Yersinia pseudotuberculosis interrupts early phosphotyrosine signalling associated with phagocytosis.
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). (Lundgren)
Vise andre og tillknytning
1996 (engelsk)Inngår i: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 20, nr 5, s. 1057-69Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The PTPase YopH of Yersinia is essential to the ability of these bacteria to block phagocytosis. Wild-type Yersinia pseudotuberculosis, but not the yopH mutant strain, resisted phagocytosis by J774 cells. Ingestion of a yopH mutant was dependent on tyrosine kinase activity. Transcomplementation with wild-type yopH restored the anti-phagocytic effect, whereas introduction of the gene encoding the catalytically inactive yopHC403A was without effect. The PTPase inhibitor orthovanadate impaired the anti-phagocytic effect of the wild-type strain, further demonstrating the importance of bacteria-derived PTPase activity for this event. The ability to resist phagocytosis indicates that the effect of the bacterium is immediately exerted when it becomes associated with the phagocyte. Within 30 s after the onset of infection, wild-type Y. pseudotuberculosis caused a YopH-dependent dephosphorylation of phosphotyrosine proteins in J774 cells. Furthermore, interaction of the cells with phagocytosable strains led to a rapid and transient increase in tyrosine phosphorylation of paxillin and some other proteins, an event dependent on the presence of the bacterial surface-located protein invasin. Co-infection with the phagocytosable strain and the wild-type strain abolished the induction of tyrosine phosphorylation. Taken together, the present findings demonstrate an immediate YopH-mediated dephosphorylation of macrophage phosphotyrosine proteins, suggesting that this PTPase acts by preventing early phagocytosis-linked signalling in the phagocyte.

sted, utgiver, år, opplag, sider
1996. Vol. 20, nr 5, s. 1057-69
Identifikatorer
URN: urn:nbn:se:umu:diva-65076PubMedID: 8809758OAI: oai:DiVA.org:umu-65076DiVA, id: diva2:603220
Tilgjengelig fra: 2013-02-05 Laget: 2013-02-05 Sist oppdatert: 2018-06-08
Inngår i avhandling
1. Interactions between Yersinia pseudotuberculosis and host cells - role of invasin and YopH
Åpne denne publikasjonen i ny fane eller vindu >>Interactions between Yersinia pseudotuberculosis and host cells - role of invasin and YopH
1999 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Umeå: Umeå universitet, 1999. s. 72
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 634
HSV kategori
Identifikatorer
urn:nbn:se:umu:diva-65079 (URN)91-7191-732-2 (ISBN)
Disputas
Föreläsningssalen, Institutionen för mikrobiologi, Umeå universitet, Umeå (engelsk)
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Veileder
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Tilgjengelig fra: 2013-02-06 Laget: 2013-02-05 Sist oppdatert: 2018-03-15bibliografisk kontrollert

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