Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Functional aspects of inorganic phosphate transport
Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
2012 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Inorganic phosphate is an essential nutrient for all organisms. It is required for many cellular components as nucleic acids and phospholipids, and as energy-carrying compounds such as ATP. Thus, a regulated uptake of this pivotal nutrient is of outermost importance. Depending of the availability of phosphate in the surroundings the yeast Saccharomyces cerevisiae make use of two different systems for transporting phosphate into the interior of the cell: a low-affinity system that is active during surplus phosphate conditions and a high-affinity system that is active when the availability becomes limited. This thesis focuses on the high-affinity system, which is comprised of the Pho84 and Pho89 transporters. Of the two transporters, Pho84 is the predominant one, responsible for almost all phosphate uptake during low phosphate conditions, and the contribution of Pho89 is of minor importance. Hence Pho84 is by far the most well characterized phosphate transporter. Even though much is known about phosphate transporters in yeast little in known about how phosphate is transported. The work in this thesis aims to broaden the knowledge about the transport mechanism by the means of site-directed mutagenesis and functional characterization. Also the similarity of Pho84 to glucose sensors and the potential role of conserved residues in phosphate signaling are investigated. By the use of a high-affinity system deletion strain (∆Pho84 ∆Pho89), we also managed to investigate the functional importance of well conserved residues in Pho89. In summary: the work presented in this thesis has contributed to increase the knowledge about transport mechanisms in phosphate transporters.

sted, utgiver, år, opplag, sider
Kalmar, Växjö: Linnaeus University Press, 2012. , s. 150
Serie
Linnaeus University Dissertations ; 94/2012
Emneord [en]
phosphate membrane transport mutagenesis saccharomyces cerevisae
HSV kategori
Forskningsprogram
Naturvetenskap, Biokemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-18651ISBN: 978-91-86983-67-3 (tryckt)OAI: oai:DiVA.org:lnu-18651DiVA, id: diva2:527515
Disputas
2012-06-15, N2007, Smålandsgatan 26B, Kalmar, 10:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2012-05-22 Laget: 2012-05-12 Sist oppdatert: 2014-02-21bibliografisk kontrollert

Open Access i DiVA

fulltext(1874 kB)412 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1874 kBChecksum SHA-512
977f7e8ccc16d874fa7a29aed282e11269a8e6669bc38c3466b899e93d639b15e3254ec034b712149ed4134d418ecb4ff9a98a5feef39273f98b2d4834db0861
Type fulltextMimetype application/pdf

Søk i DiVA

Av forfatter/redaktør
Andersson, Michael R.
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 412 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

isbn
urn-nbn

Altmetric

isbn
urn-nbn
Totalt: 418 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf