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Increased fatigue resistance linked to Ca(2+)-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut, Avdelningen för fysiologi.
Vise andre og tillknytning
2010 (engelsk)Inngår i: Journal of Physiology, ISSN 0022-3751, E-ISSN 1469-7793, Vol. 588, nr 21, s. 4275-4288Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Mammals exposed to a cold environment initially generate heat by repetitive muscle activity (shivering). Shivering is successively replaced by the recruitment of uncoupling protein-1 (UCP1)-dependent heat production in brown adipose tissue. Interestingly, adaptations observed in skeletal muscles of cold-exposed animals are similar to those observed with endurance training. We hypothesized that increased myoplasmic free [Ca2+] ([Ca2+]i) is important for these adaptations. To test this hypothesis, experiments were performed on flexor digitorum brevis (FDB) muscles, which do not participate in the shivering response, of adult wild-type (WT) and UCP1-ablated (UCP1-KO) mice kept either at room temperature (24 ºC) or cold-acclimated (4 ºC) for 4-5 weeks. [Ca2+]i (measured with indo-1) and force were measured under control conditions and during fatigue induced by repeated tetanic stimulation in intact single fibres. The results show no differences between fibres from WT and UCP1-KO mice. However, muscle fibres from cold-acclimated mice showed significant increases in basal [Ca2+]i (~50%), tetanic [Ca2+]i (~40%), and sarcoplasmic reticulum (SR) Ca2+ leak (~four-fold) as compared to fibres from room-temperature mice. Muscles of cold-acclimated mice showed increased expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and increased citrate synthase activity (reflecting increased mitochondrial content). Fibres of cold-acclimated mice were more fatigue resistant with higher tetanic [Ca2+]i and less force loss during fatiguing stimulation. In conclusion, cold exposure induces changes in FDB muscles similar to those observed with endurance training and we propose that increased [Ca2+]i is a key factor underlying these adaptations.

sted, utgiver, år, opplag, sider
2010. Vol. 588, nr 21, s. 4275-4288
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-45468DOI: 10.1113/jphysiol.2010.198598ISI: 000283718800024PubMedID: 20837639OAI: oai:DiVA.org:su-45468DiVA, id: diva2:369185
Tilgjengelig fra: 2010-11-10 Laget: 2010-11-10 Sist oppdatert: 2017-12-12bibliografisk kontrollert

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Shabalina, Irina G.Nedergaard, Jan
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