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Increased expression of protease-activated receptor-2 in mucosal mast cells in Crohns ileitis
University of Kalmar.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Kirurgi. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Kirurgi. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Kirurgi- och onkologicentrum, Kirurgiska kliniken i Östergötland med verksamhet i Linköping, Norrköping och Motala.
University of Kalmar.
2009 (engelsk)Inngår i: JOURNAL OF CROHNS and COLITIS, ISSN 1873-9946, Vol. 3, nr 2, s. 100-108Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background and aims: Activation of protease-activated receptor-2 (PAR-2) may stimulate various events of importance in inflammatory processes, including release of inflammatory mast cell mediators. PAR-2 is frequently up-regulated during inflammatory conditions, but it is not known if the expression is altered in Crohns disease. The aim of the present study was to investigate the ileal mucosal PAR-2 expression in Crohns ileitis, with particular emphasis on the expression in ileal mucosal mast cells. Methods: Surgical specimens from the distal ileum were collected from patients with Crohns ileitis and patients with colonic cancer as controls. The overall expression of PAR-2 was investigated by Western blot, and the presence of PAR-2 expressing mucosal mast cells by immunohistochemistry and cell counting. The effect of tumor necrosis factor-alpha (TNF-alpha) on the PAR-2 expression in a human mast cell tine (HMC-1) was investigated by RT-PCR and immunocytochemistry. Results: In Crohns specimens, the fraction of PAR-2-expressing mucosal. mast cells was increased about 2.5 times (P andlt; 0.001; n = 14) compared with specimens from control patients (n = 6). No difference was found between inflamed (n = 6) and uninflamed Crohns specimens (P andgt; 0.05; n = 8). Exposure to TNF-alpha for 48 h up-regulated PAR-2 mRNA and protein expression in the HMC-1 cell line. Conclusion: PAR-2 is up-regulated on ileal mucosal mast cells in Crohns ileitis, possibly due to the action of inflammatory cytokines, such as TNF-alpha. This may contribute to perpetuating the inflammatory process in the intestinal mucosa in Crohns ileitis.

sted, utgiver, år, opplag, sider
2009. Vol. 3, nr 2, s. 100-108
Emneord [en]
Crohns ileitis, Mast cells, Protease-activated receptor-2, Tumor necrosis factor-alpha
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-19533DOI: 10.1016/j.crohns.2008.11.003OAI: oai:DiVA.org:liu-19533DiVA, id: diva2:225765
Tilgjengelig fra: 2009-06-29 Laget: 2009-06-26 Sist oppdatert: 2009-08-17

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