Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The effects of PTPN2 loss on cell signalling and clinical outcome in relation to breast cancer subtype
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för Kirurgi, Ortopedi och Onkologi. Linköpings universitet, Medicinska fakulteten.ORCID-id: 0000-0002-3084-7881
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för Kirurgi, Ortopedi och Onkologi. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Diagnostikcentrum, Klinisk patologi.
Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Centrum för kirurgi, ortopedi och cancervård, Onkologiska kliniken US.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för Kirurgi, Ortopedi och Onkologi. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Centrum för kirurgi, ortopedi och cancervård, Onkologiska kliniken US.
Vise andre og tillknytning
2019 (engelsk)Inngår i: Journal of Cancer Research and Clinical Oncology, ISSN 0171-5216, E-ISSN 1432-1335, Vol. 145, nr 7, s. 1845-1856Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

PurposeThe protein tyrosine phosphatase PTPN2 dephosphorylates several tyrosine kinases in cancer-related signalling pathways and is thought to be a tumour suppressor. As PTPN2 is not frequently studied in breast cancer, we aimed to explore the role of PTPN2 and the effects of its loss in breast cancer.MethodsProtein expression and gene copy number of PTPN2 were analysed in a cohort of pre-menopausal breast cancer patients with immunohistochemistry and droplet digital PCR, respectively. PTPN2 was knocked down in three cell lines, representing different breast cancer subtypes, with siRNA transfection. Several proteins related to PTPN2 were analysed with Western blot.ResultsLow PTPN2 protein expression was found in 50.2% of the tumours (110/219), gene copy loss in 15.4% (33/214). Low protein expression was associated with a higher relapse rate in patients with Luminal A and HER2-positive tumours, but not triple-negative tumours. In vitro studies further suggested a subtype-specific role of PTPN2. Knockdown of PTPN2 had no effect on the triple-negative cell line, whilst knockdown in MCF7 inhibited phosphorylation of Met and promoted that of Akt. Knockdown in SKBR3 led to increased Met phosphorylation and decreased Erk phosphorylation as well as EGF-mediated STAT3 activation.ConclusionWe confirm previous studies showing that the PTPN2 protein is lost in half of the breast cancer cases and gene deletion occurs in 15-18% of the cases. Furthermore, the results suggest that the role of PTPN2 is subtype-related and should be further investigated to assess how this could affect breast cancer prognosis and treatment response.

sted, utgiver, år, opplag, sider
SPRINGER , 2019. Vol. 145, nr 7, s. 1845-1856
Emneord [en]
PTPN2; TCPTP; ddPCR; IHC; Breast cancer; Akt; Met; HGF
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-158849DOI: 10.1007/s00432-019-02918-yISI: 000471645800015PubMedID: 31025094OAI: oai:DiVA.org:liu-158849DiVA, id: diva2:1337647
Merknad

Funding Agencies|Swedish Cancer Society; ALF Grants, Region Ostergotland; LiU Cancer Foundation; Cancer Research Foundations of Radiumhemmet; Onkologiska Klinikernas i Linkoping Forskningsfond

Tilgjengelig fra: 2019-07-16 Laget: 2019-07-16 Sist oppdatert: 2019-11-07

Open Access i DiVA

fulltext(1586 kB)14 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1586 kBChecksum SHA-512
f92ee2e0c2d110a9640a32f7e9c837049df300533cb84798b5cf10084c6e3b10504f3b06dca8d0acf7bd9cd5665c27b9d4ec84abb3accc10df40cc2c995fac3c
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Søk i DiVA

Av forfatter/redaktør
Veenstra, CynthiaKarlsson, ElinMirwani, Sanam MirwaniNordenskjöld, BoPerez Tenorio, GizehStål, Olle
Av organisasjonen
I samme tidsskrift
Journal of Cancer Research and Clinical Oncology

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 14 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 26 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf