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Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.ORCID-id: 0000-0002-5249-4415
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.ORCID-id: 0000-0002-3049-6831
Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Tekniska sektionen, Institutionen för teknikvetenskaper, Fasta tillståndets fysik.ORCID-id: 0000-0003-0648-3130
Vise andre og tillknytning
2018 (engelsk)Inngår i: ACS Sensors, ISSN 2379-3694, Vol. 3, nr 6, s. 1093-1101Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The ability to detect and analyze the state ofmagnetic labels with high sensitivity is of crucial importance fordeveloping magnetic biosensors. In this work, we demonstrate, forthefirst time, a ferromagnetic resonance (FMR) basedhomogeneous and volumetric biosensor for magnetic labeldetection. Two different isothermal amplification methods, i.e.,rolling circle amplification (RCA) and loop-mediated isothermalamplification (LAMP), are adopted and combined with a standardelectron paramagnetic resonance (EPR) spectrometer for FMRbiosensing. For the RCA-based FMR biosensor, binding of RCAproducts of a syntheticVibrio choleraetarget DNA sequence givesrise to the formation of aggregates of magnetic nanoparticles.Immobilization of nanoparticles within the aggregates leads to adecrease of the net anisotropy of the system and a concomitant increase of the resonancefield. A limit of detection of 1 pM isobtained with a linear detection range between 7.8 and 250 pM. For the LAMP-based sensing, a synthetic Zika virus targetoligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by theircoprecipitation with Mg2P2O7(a byproduct of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement,high sensitivity, and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate fordesigning future point-of-care devices.

sted, utgiver, år, opplag, sider
2018. Vol. 3, nr 6, s. 1093-1101
HSV kategori
Forskningsprogram
Teknisk fysik med inriktning mot fasta tillståndets fysik
Identifikatorer
URN: urn:nbn:se:uu:diva-345595DOI: 10.1021/acssensors.8b00048ISI: 000436525800005OAI: oai:DiVA.org:uu-345595DiVA, id: diva2:1189363
Forskningsfinansiär
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 2011-1692EU, FP7, Seventh Framework Programme, FP7-NMP-601118Tilgjengelig fra: 2018-03-09 Laget: 2018-03-09 Sist oppdatert: 2019-04-24bibliografisk kontrollert
Inngår i avhandling
1. Magnetic Nanoparticle Based Biosensors for Pathogen Detection and Cancer Diagnostics
Åpne denne publikasjonen i ny fane eller vindu >>Magnetic Nanoparticle Based Biosensors for Pathogen Detection and Cancer Diagnostics
2018 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis describes several magnetic nanoparticle (MNP)-based biosensing strategies which take advantage of different magnetic sensors, molecular tools and nanotechnologies. Proposed biosensors can be classified into three groups, i.e., immunoassay-based, molecular amplification-based, and nanoparticle assembly-based. The principal motivation is to develop and optimize biosensors for out-of-lab and point-of-care testing.

Immunoassay-based biosensors described in this thesis employ antibodies as the bio-recognition element for the detection of bacteria cells/fragments or proteins. Two typical immunoassay formats, i.e., direct and competitive format, are studied and compared for bacteria detection. In addition, in the protein biomarker detection, MNP chains are formed in the presence of target analytes as well as in the external rotating magnetic field. The high shape/magnetic anisotropy of the chains provides better optomagnetic performance.

Two different molecular amplification methods, i.e., rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP), are described under the topic of molecular amplification-based biosensors. In RCA-based biosensors, DNA probe modified MNPs bind to the amplicons after amplification. In LAMP-based biosensors, MNPs are either modified with primers that keep growing during the amplification, or are co-precipitated with the by-product (Mg2P2O7) of the amplification.

The design of the nanoparticle assembly-based biosensors described in this thesis is based on duplex-specific nuclease (DSN)-assisted target recycling and core-satellite magnetic superstructures. In the presence of target microRNA, DSN cuts the DNA scaffold of the core-satellite assembly, releasing MNP satellites that can be quantified by the sensor.

Different kinds of target analytes, i.e., pathogens or cancer biomarkers, are detected at the aiming for rapid, low-cost and user-friendly diagnostics.

sted, utgiver, år, opplag, sider
Uppsala: Acta Universitatis Upsaliensis, 2018. s. 55
Serie
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1647
Emneord
Magnetic biosensors, magnetic nanoparticles, homogeneous assays, volumetric sensing
HSV kategori
Forskningsprogram
Teknisk fysik med inriktning mot fasta tillståndets fysik
Identifikatorer
urn:nbn:se:uu:diva-346014 (URN)978-91-513-0278-2 (ISBN)
Disputas
2018-05-04, Häggsalen, Ångströmlaboratoriet, Lägerhyddsv. 1, Uppsala, 13:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2018-04-13 Laget: 2018-03-13 Sist oppdatert: 2018-04-24

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