Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Preparation of Affinity Based Probes to Elucidate the Mode of Action of y-Secretase Modulators
KTH, Skolan för kemivetenskap (CHE).
2011 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)Alternativ titel
Syntes av affinitetsbaserade ligander för att undersöka funktionen hos y-sekretasmodulatorer (Svenska)
Abstract [en]

γ-Secretase is an enzyme that takes part of the breakdown of amyloid precursor protein (APP) into amyloid-β (Aβ) peptides. Aβ-peptides from this process can be linked to Alzheimer’s disease. γ-Secretase modulators (GSMs) have been reported in the literature to reduce the potentially dangerous variant of Aβ-peptides. However, the mechanism of the binding site for these modulators is poorly understood. In this report substances of affinity based probes (AfBPs) have been prepared that can be used as a method to extract the binding site. The AfBPs were constituted of a cross-linking group, a biotinyl group and a γ-secretase modulator. Different variants of the biotin probe were prepared to investigate if the activity of the original modulator could be retained. The report will show that this could be done.

In the future, the synthesised affinity based probes could be used to understand the mode of action of γ-secretase modulators. For instance the modulator could be incubated with the enzyme and the crosslinking group, when activated, covalently bind to a residue near the binding site. The complex could then be captured on avidin and the binding site be determined.

Ort, förlag, år, upplaga, sidor
2011. , s. 30
Nyckelord [en]
Alzheimer’s disease (AD), γ-Secretase modulator (GSM), affinity based probe (AfBP), biotinyl, amyloid precursor protein (APP)
Nationell ämneskategori
Organisk kemi
Identifikatorer
URN: urn:nbn:se:kth:diva-51162OAI: oai:DiVA.org:kth-51162DiVA: diva2:463572
Ämne / kurs
Organisk kemi
Utbildningsprogram
Civilingenjörsexamen - Kemivetenskap
Uppsök
fysik/kemi/matematik
Handledare
Examinatorer
Tillgänglig från: 2012-02-27 Skapad: 2011-12-09 Senast uppdaterad: 2012-02-27Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Av organisationen
Skolan för kemivetenskap (CHE)
Organisk kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 9 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf