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Cyclotide Evolution: Insights from the Analyses of Their Precursor Sequences, Structures and Distribution in Violets (Viola)
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.ORCID-id: 0000-0003-0754-1566
Kangwon National University, Department of Biological Sciencesi.
University of Oslo, Centre for Ecological and Evolutionary Synthesis, Department of Biosciences.
Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Farmaceutiska fakulteten, Institutionen för läkemedelskemi, Avdelningen för farmakognosi.
Vise andre og tillknytning
2017 (engelsk)Inngår i: Frontiers in Plant Science, ISSN 1664-462X, E-ISSN 1664-462X, Vol. 8, artikkel-id 2058Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Cyclotides are a family of plant proteins that are characterized by a cyclic backbone and a knotted disulfide topology. Their cyclic cystine knot (CCK) motif makes them exceptionally resistant to thermal, chemical, and enzymatic degradation. By disrupting cell membranes, the cyclotides function as host defense peptides by exhibiting insecticidal, anthelmintic, antifouling, and molluscicidal activities. In this work, we provide the first insight into the evolution of this family of plant proteins by studying the Violaceae, in particular species of the genus Viola. We discovered 157 novel precursor sequences by the transcriptomic analysis of six Viola species: V. albida var. takahashii, V. mandshurica, V. orientalis, V. verecunda, V. acuminata, and V. canadensis. By combining these precursor sequences with the phylogenetic classification of Viola, we infer the distribution of cyclotides across 63% of the species in the genus (i.e., ~380 species). Using full precursor sequences from transcriptomes, we show an evolutionary link to the structural diversity of the cyclotides, and further classify the cyclotides by sequence signatures from the non-cyclotide domain. Also, transcriptomes were compared to cyclotide expression on a peptide level determined using liquid chromatography-mass spectrometry. Furthermore, the novel cyclotides discovered were associated with the emergence of new biological functions.

sted, utgiver, år, opplag, sider
2017. Vol. 8, artikkel-id 2058
Emneord [en]
cyclotide evolution, viola phylogeny, sequence signature, cyclotide precursor, neofunctionality, novel cyclotide, precursor domain
HSV kategori
Identifikatorer
URN: urn:nbn:se:uu:diva-339782DOI: 10.3389/fpls.2017.02058ISI: 000418117800001PubMedID: 29326730OAI: oai:DiVA.org:uu-339782DiVA: diva2:1181854
Forskningsfinansiär
Swedish Research Council, 2012-5063
Tilgjengelig fra: 2018-02-09 Laget: 2018-02-09 Sist oppdatert: 2018-02-09bibliografisk kontrollert

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