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  • Peixoto, Tiago P.
    et al.
    Rosvall, Martin
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
    Modelling sequences and temporal networks with dynamic community structures2017Inngår i: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 8, 582Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    In evolving complex systems such as air traffic and social organisations, collective effects emerge from their many components' dynamic interactions. While the dynamic interactions can be represented by temporal networks with nodes and links that change over time, they remain highly complex. It is therefore often necessary to use methods that extract the temporal networks' large-scale dynamic community structure. However, such methods are subject to overfitting or suffer from effects of arbitrary, a priori-imposed timescales, which should instead be extracted from data. Here we simultaneously address both problems and develop a principled data-driven method that determines relevant timescales and identifies patterns of dynamics that take place on networks, as well as shape the networks themselves. We base our method on an arbitrary-order Markov chain model with community structure, and develop a nonparametric Bayesian inference framework that identifies the simplest such model that can explain temporal interaction data.

  • Christianson, Helena C.
    et al.
    Menard, Julien A.
    Chandran, Vineesh Indira
    Bourseau-Guilmain, Erika
    Shevela, Dmitry
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
    Lidfeldt, Jon
    Mansson, Ann-Sofie
    Pastorekova, Silvia
    Messinger, Johannes
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Skane Univ Hosp, Lund, Sweden.
    Belting, Mattias
    Tumor antigen glycosaminoglycan modification regulates antibody-drug conjugate delivery and cytotoxicity2017Inngår i: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 8, nr 40, 66960-66974 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Aggressive cancers are characterized by hypoxia, which is a key driver of tumor development and treatment resistance. Proteins specifically expressed in the hypoxic tumor microenvironment thus represent interesting candidates for targeted drug delivery strategies. Carbonic anhydrase (CAIX) has been identified as an attractive treatment target as it is highly hypoxia specific and expressed at the cell-surface to promote cancer cell aggressiveness. Here, we find that cancer cell internalization of CAIX is negatively regulated by post-translational modification with chondroitin or heparan sulfate glycosaminoglycan chains. We show that perturbed glycosaminoglycan modification results in increased CAIX endocytosis. We hypothesized that perturbation of CAIX glycosaminoglycan conjugation may provide opportunities for enhanced drug delivery to hypoxic tumor cells. In support of this concept, pharmacological inhibition of glycosaminoglycan biosynthesis with xylosides significantly potentiated the internalization and cytotoxic activity of an antibody-drug conjugate (ADC) targeted at CAIX. Moreover, cells expressing glycosaminoglycan-deficient CAIX were significantly more sensitive to ADC treatment as compared with cells expressing wild-type CAIX. We find that inhibition of CAIX endocytosis is associated with an increased localization of glycosaminoglycan-conjugated CAIX in membrane lipid raft domains stabilized by caveolin-1 clusters. The association of CAIX with caveolin-1 was partially attenuated by acidosis, i.e. another important feature of malignant tumors. Accordingly, we found increased internalization of CAIX at acidic conditions. These findings provide first evidence that intracellular drug delivery at pathophysiological conditions of malignant tumors can be attenuated by tumor antigen glycosaminoglycan modification, which is of conceptual importance in the future development of targeted cancer treatments.

  • Wagman, Petra
    et al.
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för rehabilitering. Högskolan i Jönköping, Hälsohögskolan, HHJ. ADULT.
    Björklund, Anita
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för rehabilitering. Högskolan i Jönköping, Hälsohögskolan, HHJ. ADULT. Högskolan i Jönköping, Hälsohögskolan, HHJ. ARN-J (Aging Research Network - Jönköping).
    Johansson, Ann
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för rehabilitering. Högskolan i Jönköping, Hälsohögskolan, HHJ. ARN-J (Aging Research Network - Jönköping).
    Fristedt, Sofi
    Högskolan i Jönköping, Hälsohögskolan, HHJ, Avd. för rehabilitering. Högskolan i Jönköping, Hälsohögskolan, HHJ. ARN-J (Aging Research Network - Jönköping). Högskolan i Jönköping, Hälsohögskolan, HHJ. IMPROVE (Improvement, innovation, and leadership in health and welfare).
    Descriptions of health by EU citizens begging abroad2017Inngår i: Society, health and vulnerability, ISSN 0283-1929, E-ISSN 1751-9020, Vol. 8, nr 1, 1389586Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Poor citizens from European Union (EU) member countries begging to support themselves are now common in affluent EU countries. Their lack of basic amenities, such as access to a shelter and sanitation is not in line with human rights and also implies a risk for health issues. Despite this, we know little about how these vulnerable EU citizens, themselves, perceive their health situation. The aim of this study was to explore vulnerable EU citizens’ descriptions of their health. Eight females and 12 males from Romania, 19–64 years of age, participated in individual interviews that included health issues. Qualitative content analysis was used, identifying the main category “Begging abroad and health - for better and for worse” together with two categories and five subcategories. It is concluded that EU citizens begging abroad risk poorer health as a consequence of their attempts to improve their situation, both their own health and that of their relatives. Therefore, they need access to affordable health care as this may decrease the need for unwanted travelling abroad to beg.

  • Dancila, Dragos
    et al.
    Uppsala Universitet.
    Beuerle, Bernhard
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shah, Umer
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Rydberg, Anders
    Uppsala Universitet.
    Oberhammer, Joachim
    Micromachined Cavity Resonator Sensors for on Chip Material Characterisation in the 220–330 GHz band2017Inngår i: Proceedings of the 47th European Microwave Conference, Nuremberg, October 8-13, 2017, 2017Konferansepaper (Fagfellevurdert)
    Abstract [en]

    A silicon micromachined waveguide on-chip sensor for J-band (220-325 GHz) is presented. The sensor is based on a micromachined cavity resonator provided with an aperture in the top side of a hollow waveguide for sensing purposes. The waveguide is realized by microfabrication in a silicon wafer, goldmetallized and assembled by thermocompression bonding. The sensor is used for measuring the complex relative permittivity of different materials. Preliminary measurements of several dielectric materials are performed, demonstrating the potential of the sensor and methodology.

  • Beuerle, Bernhard
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Campion, James
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Shah, Umer
    KTH, Skolan för elektro- och systemteknik (EES), Mikro- och nanosystemteknik.
    Oberhammer, Joachim
    Integrated Micromachined Waveguide Absorbers at 220 – 325 GHz2017Inngår i: Proceedings of the 47th European Microwave Conference, Nuremberg, October 8-13, 2017, 2017Konferansepaper (Fagfellevurdert)
    Abstract [en]

    This paper presents the characterization of integrated micromachined waveguide absorbers in the frequency band of 220 to 325 GHz. Tapered absorber wedges were cut out of four different commercially available semi-rigid absorber ma terials and inserted in a backshorted micromachined waveguide cavity for characterization. The absorption properties of these materials are only specified at 10 GHz, and their absorption behavior above 100 GHz was so far unknown. To study the effect of the geometry of the absorber wedges, the return loss of different absorber lengths and tapering angles was investigated. The results show that longer and sharper sloped wedges from the material specified with the lowest dielectric constant, but not the highest specified absorption, are superior over other geometries and absorber materials. The best results were achieved for 5 mm long absorbers with a tapering angle of 23° in the material RS-4200 from the supplier Resin Systems, having a return loss of better than 13 dB over the whole frequency range of 220 to 325 GHz. These absorber wedges are intended to be used as matched loads in micromachined waveguide circuits. To the best of our knowledge, this is the first publication characterizing such micromachined waveguide absorbers.

  • Karlsson, Jessica
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    Gouveia-Figueira, Sandra
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Swedish Univ Agr Sci, Umea, Sweden.
    Alhouayek, Mireille
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    Fowler, Christopher J.
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    Effects of tumour necrosis factor alpha upon the metabolism of the endocannabinoid anandamide in prostate cancer cells2017Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, nr 9, e0185011Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Tumour necrosis factor a (TNF alpha) is involved in the pathogenesis of prostate cancer, a disease where disturbances in the endocannabinoid system are seen. In the present study we have investigated whether treatment of DU145 human prostate cancer cells affects anandamide (AEA) catabolic pathways. Additionally, we have investigated whether cyclooxygenase- 2 (COX-2) can regulate the uptake of AEA into cells. Levels of AEA synthetic and catabolic enzymes were determined by qPCR. AEA uptake and hydrolysis in DU145 and RAW264.7 macrophage cells were assayed using AEA labeled in the arachidonic and ethanolamine portions of the molecule, respectively. Levels of AEA, related N-acylethanolamines (NAEs), prostaglandins (PG) and PG-ethanolamines (PG-EA) in DU145 cells and medium were quantitated by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. TNF alpha treatment of DU145 cells increased mRNA levels of PTSG2 (gene of COX-2) and decreased the mRNA of the AEA synthetic enzyme N-acylphosphatidylethanolamine selective phospholipase D. mRNA levels of the AEA hydrolytic enzymes fatty acid amide hydrolase (FAAH) and N-acylethanolamine-hydrolyzing acid amidase were not changed. AEA uptake in both DU145 and RAW264.7 cells was inhibited by FAAH inhibition, but not by COX-2 inhibition, even in RAW264.7 cells where the expression of this enzyme had greatly been induced by lipopolysaccharide + interferon. treatment. AEA and related NAEs were detected in DU145 cells, but PGs and PGE(2)-EA were only detected when the cells had been preincubated with 100 nM AEA. The data demonstrate that in DU145 cells, TNFa treatment changes the relative expression of the enzymes involved in the hydrolytic and oxygenation catabolic pathways for AEA. In RAW264.7 cells, COX-2, in contrast to FAAH, does not regulate the cellular accumulation of AEA. Further studies are necessary to determine the extent to which inflammatory mediators are involved in the abnormal endocannabinoid signalling system in prostate cancer.

  • Reiz, Sebastian
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Anestesiologi och intensivvård.
    Hemodynamic and cardiometabolic studies in patients with distributive circulatory dysfunctions: with special reference to the effects of the beta-1-adrenoreceptor agonist prenalterol1979Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    A total of 49 patients were studied, using invasive hemodynamic techniques with systemic arterial, pulmonary artery and right atrial pressure recordings together with thermodilution cardiac output determinations. Sixteen of the patients were also subjected to cardiometabolic studies, using measurement of coronary sinus blood flow by the continuous thermodilution technique and analyses of oxygen content and lactate concentration in the systemic and coronary circulation. A common denominator in the five investigations was, that a distributive cardiovascular dysequilibrium was either induced (for surgical or anaesthesiological reasons) or already present due to a pathological condition.

    Thoracic epidural block from T 1 to T 12 induced marked decrease in systemic blood pressure due to vasodilation and impairment of cardiac performance. Prenalterol administration effectively abolished the low blood pressure by its marked inotropic action, having no effect on systemic vascular resistance. Myocardial oxygen consumption changed in parallel with the changes in cardiac work following both thoracic epidural block and prenalterol. Coronary vascular resistance was markedly decreased by the block and was not affected by prenalterol. It is suggested, that the critically low perfusion pressure is the main cause of the coronary vasodilation and that alpha-blockade induced by the thoracic epidural block is of less importance.

    The combination of a thoracic epidural block from T 1 to T 12 and selective ßi-stimulation with prenalterol was an effective way to modify the cardiovascular response to infrarenal aortic cross clamping. This treatment transferred the patients to a more favourable cardiac function curve and possibly facilitated the redistribution of blood flow in association with clamping.

    In association with declamping of the infrarenal aorta or the common iliac arteries, volume loading to a slightly elevated left ventricular filling pressure shortly before declamping was an effective way to counteract the expected blood pressure drop. A normal left ventricular filling pressure prior to declamping did not prevent the blood pressure drop following declamping. It is suggested, that mismatching between vascular volume and blood volume is the main cause of declamping hypotension.

    In patients with low resistance, distributive septic shock caused by gram negative bacteremias and signs of impaired cardiac function, prenalterol effectively reversed the hypotension and improved tissue perfusion by selectively increasing cardiac output. In parallel to the increased cardiac work, an increase in myocardial metabolic demand was demonstrated.

  • Edlund, Ulf
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
    Studier av 2-cykloaminoindener speciellt med hänsyn till prototropa omlagringar1974Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    This thesis is a short review of six papers (Papers 1-6,page 5) concerning studies of enamines from 2-indanones. The unusuallystable 2-(N-pyrrolidyl)indene has been studied (Paper 2 ). As a base catalyst this enamine rearranges 1-methylindene to 3-methylindene with a lower rate constant compared with triethylamine. During this 1 ,3-prototropic rearrangement no hydrogen exchangewas observed (Paper 1).

    Special attention has been drawn to 1 ,3-rearrangements ofalkyl-substituted enamines of 2-indanone. Prom 1-methyl-2-indanone, which was most conveniently prepared by oxidation of the correspondingindene, a series of enamines has been prepared with varyingamine components. A study of the kinetic control of the formation of these enamines showed that the less substituted double-bondisomer was formed more rapidly than the other form. Furthermore, the nature of the amine group has a significant influence on the composition of the equilibrium mixture observed, but the isomericratios obtained were unexpected in the light of earlier investigationsof enamines from 2-methylcyclohexanone (Paper l).

    A 13C NMR study of these 2-cycloaminoindenes showed that introduction of a 1-methyl substituent caused a decrease in overlap but the magnitude of this perturbation was almost independent of the variation of the amine component. Furthermore, when using pyrrolidineor hexamethyleneimine as amine parts this decrease in overlapwas of a comparable magnitude with the diminished conjugationnoted in the 3-methyl isomers. However, when piperidine or morpholinewas used the decreased delocalization was more pronounced inthe 3-methyl tautomers compared with the 1-methyl analogues (Paper6).

    One isomer, 1-methyl-2-(N-piperidyl)indene, has been shown toundergo base- as well as acid-catalyzed 1,3-tautomerizations inpyridine solvent. The isomerization rates using 1,4-diazabicyclo-(2.2.2.)octane or quinuclidine as base catalysts were considerably slower than the rate of the acid-catalyzed reaction or the basecatalyzedrearrangement rates involving alkyl-substituted indenesas substrates. This retarding effect, caused by the amine group, is in agreement with differences in pKA values obtained via simpleHückel calculation (Paper 4).

    The reaction between pyrrolidine and 1-(2-indanylidene)indan--2-one has been investigated. Two possible mechanisms were sugges-ted for the formation of products consisting of a linear dienamineand an indenyl-substituted enamine (9:1). In the main productisolated by recrystallization, the indenyl substituent was shownto be twisted out of conjugation. This dienamine also underwentacid-catalyzed isomerization to form the enamine product (Paper 3).

    Convenient syntheses of the pharmacologically interestingN-substituted 2-aminoindanes have also been described. The perchloratesalts of the enamines were treated with sodium borohydride in methanol to afford the desired products (Paper 5).

  • Hagner, Mats
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Medicinsk och klinisk genetik.
    Experimentell bestämning av klimatanpassning hos tall (Pinus silvestris L. och Pinus contorta Dougl.) = Experimental assessment of climatic adaptation of pine1970Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    This paper summarizes the content of three articles published in Studia Forestalia Suecica 80,81,82, 1970.

    A study of the annual grouuth rhythm on 2-, 3- and 20-year-old Pinus silvestris and 2-year-old Pinus contorta showed that the following methods can be used for experimental assessment of the climatic adaptation of populations and single trees: shoot- and needle elongation, bark colour onterminal, lignification of annual ring and water content of needles.

    It was established that the variation in rhythm within a population is so large that a portion of the trees within a stand progeny cannot be considered as adapted to the environment in which they were born.

    The annual rhythm was found to be correlated with the height growth of the single tree and with the survival, frost resistance and height growth of the population. It was shown that the regression of height on rhythm within a population may be used for assessment of the degree of adaptation to the testing environment. In tree breeding registration of the annual rhythm would improve the prediction of what environment a progeny is best adapted to and increase the precision in the evaluation of the potential growth capacity of the progeny.

  • Solheim, Folke
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Obstetrik och gynekologi.
    Blood loss at first trimester abortion by means of vacuum aspiration1976Doktoravhandling, med artikler (Annet vitenskapelig)
  • Carstedt, Göran
    et al.
    Umeå universitet, Samhällsvetenskapliga fakulteten, Handelshögskolan vid Umeå universitet, Företagsekonomi.
    Isaksson Pérez, Birgitta
    Umeå universitet, Samhällsvetenskapliga fakulteten, Handelshögskolan vid Umeå universitet, Företagsekonomi.
    Företag i strukturomvandlingen. D. 5, Summary1974Doktoravhandling, monografi (Annet vitenskapelig)
  • Lindgren, Hanna
    et al.
    Statens väg- och transportforskningsinstitut, Samhälle, miljö och transporter, SAMT, Transportekonomi, TEK.
    Berg, Jessica
    Statens väg- och transportforskningsinstitut, Samhälle, miljö och transporter, SAMT, Mobilitet, aktörer och planering, MAP.
    Lösningar för kollektivtrafik på landsbygd: det pågående arbetet i Sverige2017Rapport (Annet vitenskapelig)
    Abstract [sv]

    VTI har fått i uppdrag av regeringen att genomföra en studie om effektiva och innovativa lösningar som kan öka medborgarnas vilja och möjlighet att utnyttja kollektivtrafiken på landsbygd. Denna studie är en delstudie inom uppdraget som syftar till att identifiera goda exempel på lösningar. Ytterligare ett syfte är att studera de regionala kollektivtrafikmyndigheternas arbete med att identifiera lösningar som är både attraktiva för resenärer och ekonomiskt effektiva. För att besvara studiens syften används tre empiriska källor: enkätstudie riktad till regionala kollektivtrafikmyndigheter, intervjuer med tjänstemän på fyra regionala kollektivtrafikmyndigheter samt en studie av lösningar och projekt som har bedrivits och bedrivs i Sverige. I intervjustudien ska följande frågor besvaras:

    1. Vilka utmaningar och förutsättningar för kollektivtrafik på landsbygd har de regionala kollektivtrafikmyndigheterna? Vilka förutsättningar har de för att lösa eventuella problem?

    2. Har de regionala kollektivtrafikmyndigheterna några strategier för att bedriva kollektivtrafik på landsbygd? I så fall, hur ser strategierna ut?

    3. Hur går den organisatoriska och ekonomiska samordningen av olika transportformer till?

    Resultaten visar att landsbygden ofta underprioriteras i kollektivtrafikplaneringen. Satsningar görs där man kan attrahera många resenärer. Knappa ekonomiska resurser resulterar i att tätortstrafik prioriteras medan höga kostnader och låg finansieringsgrad är orsaker till att projekt som har testats tvingas att lägga ned. Den anropsstyrda trafiken kan bli bättre. Idag används till exempel digitala lösningar i mycket liten omfattning. I samåkningsprojekt visas att intresset för samåkning har varit lågt. För att öka tillgängligheten på landsbygd behöver kombinerade tjänster för service såsom inköp, postservice och sjukvård och olika former av mobilitetstjänster införas.

  • Chabuk, Ali
    et al.
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Geoteknologi. Department of Environment Engineering, College of Engineering, University of Babylon.
    Al-Ansari, Nadhir
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Geoteknologi.
    Hussain, Hussain Musa
    Department of Geology, Faculty of Science, University of Kufa.
    Knutsson, Sven
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Geoteknologi.
    Pusch, Roland
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Geoteknologi.
    Laue, Jan
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Geoteknologi.
    Combining GIS Applications and Method of Multi-Criteria Decision-Making (AHP) for Landfill Siting in Al-Hashimiyah Qadhaa, Babylon, Iraq2017Inngår i: Sustainability, ISSN 2071-1050, E-ISSN 2071-1050, Vol. 9, nr 11, 1932Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Landfill siting is a complex process. It is one of the major problems in waste management, where many factors should be taken into consideration when selecting a suitable site for landfill in any given area. At the present time, there are many random waste disposal sites distributed throughout Al-Hashimiyah Qadhaa in Iraq. In this study, the Geographic Information System (GIS) and the Analytical Hierarchy Process (AHP) were used to select the best sites for landfill. The process of selecting sites for landfill in Al-Hashimiyah Qadhaa comprised two steps. First, fifteen different criteria were mapped and incorporated into overlay analyses within GIS software to produce the final suitability index map for the site. The second step comprises the exclusion of unsuitable areas from the final map to simplify identification of the candidate sites for landfill in the study area. The weightings of criteria were identified using AHP, and the weightings of the sub-criteria of each criterion were determined based on multiple factors. In order to accommodate solid waste from 2020 until 2030, two suitable candidate landfill sites were determined which fulfill the required area of 1.013 km2 with areas of 1.374 km2 and 1.288 km2 respectively.

  • Pitarokoilis, Antonios
    et al.
    School of Electrical Engineering and ACCESS Linnaeus Center, KTH Royal Institute of Technology, Stockholm, Sweden.
    Björnson, Emil
    Linköpings universitet, Institutionen för systemteknik, Kommunikationssystem. Linköpings universitet, Tekniska fakulteten.
    Larsson, Erik G
    Linköpings universitet, Institutionen för systemteknik, Kommunikationssystem. Linköpings universitet, Tekniska fakulteten.
    On the effect of imperfect timing synchronization on pilot contamination2017Inngår i: 2017 IEEE International Conference on Communications (ICC) / [ed] Debbah M.,Gesbert D.,Mellouk A., IEEE, 2017Konferansepaper (Fagfellevurdert)
    Abstract [en]

    The phenomenon of pilot contamination (PC) in multi-cell Massive MIMO systems is investigated in the presence of imperfect timing synchronization (TS). In particular, a basic setup is considered, where a base station (BS) is perfectly synchronized with the user of its cell, but there is imperfect TS between the BS and the user in another cell, possibly due to different propagation distances. A discrete-time system model is derived based on the continuous-time system model. The discrete-time system model accurately captures the phenomenon of imperfect TS in terms of the timing mismatch and the pulse shaping filter impulse responses. The derived discrete-time system model is used to study the achievable rates of a two-cell Massive MIMO uplink. It is shown that the structure imposed to the pilot contaminating signal due to the imperfect TS can be leveraged to mitigate the effect of PC. The level of PC suppression is quantified as a function of the timing mismatch and the characteristics of the transmit/receive pulse shaping filters.

  • Carstedt, Göran
    et al.
    Umeå universitet, Samhällsvetenskapliga fakulteten, Handelshögskolan vid Umeå universitet, Företagsekonomi.
    Isaksson Pérez, Birgitta
    Umeå universitet, Samhällsvetenskapliga fakulteten, Handelshögskolan vid Umeå universitet, Företagsekonomi.
    Företag i strukturomvandlingen1974Doktoravhandling, monografi (Annet vitenskapelig)
  • Hägg, Erik
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
    On the pathogenesis of glomerular lesions in the alloxan diabetic rat: a light microscopic, immunofluorescent and ultrastructural study, including the effects of insulin treatment and immunosuppression1974Doktoravhandling, med artikler (Annet vitenskapelig)
  • Melldahl, Andreas
    Uppsala universitet, Humanistisk-samhällsvetenskapliga vetenskapsområdet, Fakulteten för utbildningsvetenskaper, Institutionen för pedagogik, didaktik och utbildningsstudier.
    Modes of reproduction in the Swedish economic elite: education strategies of the children of the top one per cent2017Inngår i: European Societies: The Official Journal of the European Sociological Association, ISSN 1461-6696, E-ISSN 1469-8307, 1-29 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Most sociological studies on the economic elite have focused on groups holding formal power in the economic field. In this paper, the Swedish economic elite is constructed in line with Thomas Piketty’s notions on the richest top one per cent, but the scope extended beyond the economic analysis by combining it with a sociological perspective. Differences within this distributional group are found in the volume and composition of economic capital – as well as in other forms of capital. The elite is structured by an opposition between, first, those holding large wealth and those receiving high-wage incomes and, second, between the established elite and newcomers. Moreover, many elite studies pay attention to the routes to elite position. This paper also examines modes of reproduction from elite positions, using the education strategies employed by children of the economic elite within Swedish higher education. Such strategies are more important – and narrow – for the children of the high-income elite, revealed by their overrepresentation at certain highly selective educational institutions.

  • Andersson, Sofie
    et al.
    KTH, Skolan för elektro- och systemteknik (EES), Centra, ACCESS Linnaeus Centre. KTH, Skolan för datavetenskap och kommunikation (CSC), Centra, Centrum för Autonoma System, CAS.
    Nikou, Alexandros
    KTH, Skolan för elektro- och systemteknik (EES), Centra, ACCESS Linnaeus Centre. KTH, Skolan för datavetenskap och kommunikation (CSC), Centra, Centrum för Autonoma System, CAS.
    Dimarogonas, Dimos V.
    KTH, Skolan för elektro- och systemteknik (EES), Centra, ACCESS Linnaeus Centre. KTH, Skolan för datavetenskap och kommunikation (CSC), Centra, Centrum för Autonoma System, CAS.
    Control Synthesis for Multi-Agent Systems under Metric Interval Temporal Logic Specifications2017Inngår i: IFAC-PapersOnLine, Elsevier, 2017, Vol. 50, 2397-2402 s.Konferansepaper (Fagfellevurdert)
    Abstract [en]

    This paper presents a framework for automatic synthesis of a control sequence for multi-agent systems governed by continuous linear dynamics under timed constraints. First, the motion of the agents in the workspace is abstracted into individual Transition Systems (TS). Second, each agent is assigned with an individual formula given in Metric Interval Temporal Logic (MITL) and in parallel, the team of agents is assigned with a collaborative team formula. The proposed method is based on a correct-by-construction control synthesis method, and hence guarantees that the resulting closed-loop system will satisfy the desired specifications. The specifications considers boolean-valued properties under real-time bounds. Extended simulations has been performed in order to demonstrate the efficiency of the proposed methodology.

  • Sehlin, Janove
    Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Histologi med cellbiologi.
    Studies on the mechanism of amino acidinduced insulin release1971Doktoravhandling, med artikler (Annet vitenskapelig)
  • Berlina, Anna
    et al.
    Nordiska ministerrådet, Nordregio.
    Tepecik Diş, Asli
    Nordiska ministerrådet, Nordregio.
    Jungsberg, Leneisja
    Nordiska ministerrådet, Nordregio.
    LOCAL FOOD SYSTEMS FORMATION: The potential of local food initiatives in the Baltic Sea Region2017Rapport (Annet vitenskapelig)
    Abstract [en]

    This working paper describes the state of play of local food initiatives in the Baltic Sea Region (BSR) by examining EU and national policy contexts and by highlighting good practices of local food initiatives in Latvia, Lithuania, Poland, Sweden and Belarus. The working paper investigates the key drivers and factors impeding the development of these initiatives. The working paper is based on desk studies, input received during meetings with stakeholders and researchers from the BSR, and interviews with good practice initiators in 2016–17. This working paper is one output of the Local food: Formation of local food markets project financed by the Swedish Institute. The overall aim of the project was to strengthen co-operation and to build knowledge of local food system formation by various actors working on rural development issues in the Baltic Sea Region (BSR). Another objective of the project was to investigate and share good practices in building, shaping, reproducing and promoting alternative food networks and markets over time and space in the BSR countries (Sweden, Latvia, Lithuania, Poland and Belarus).

  • Finnström, Orvar
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Pediatrik.
    Studies on maturity in newborn infants1971Doktoravhandling, med artikler (Annet vitenskapelig)
  • Bergman, Birgitta
    Umeå universitet, Medicinska fakulteten, Institutionen för samhällsmedicin och rehabilitering.
    Being a physiotherapist: professional role, utilization of time an d vocational strategies1989Doktoravhandling, monografi (Annet vitenskapelig)
    Abstract [en]

    In a research series carried out between 1984 and 1988 in the county of Västerbotten in northern Sweden, various aspects of the professional role and work of physiotherapists were studied. A variety of research methods were used: questionnaires (n = 163), a time budget study (n = 149), and a qualitative interview (n = 24). Physiotherapy was considered varied and creative, but not well defined or very specific in its objectives. Physiotherapy is still a predominantly female profession, though the proportion of male physiotherapists was increasing. The proportion entering full-time employment in physiotherapy increased due both to the greater number of male graduates and the increasing number of women working full-time. A partial internal division of work between the sexes has arisen. More women than men are employed in in-patient care, while proportionately more men worked outside institutions. Most respondents were firmly in control of their treatment methods, but were somewhat restricted in their freedom to decide whom to treat, and when to terminate treatment. Few had carried out any research concerning treatment and results. The time budget study showed that the treatment of patients took up on average 33% of the physiotherapists’ gross working hours and was the largest single task. Continuing education accounted for 5%, development work for 1% and the remaining occupational tasks for 38%. Occupational area was the most important factor in explaining the distribution of working hours, when other factors were kept constant. Neither sex nor gender markedly affects the carrying out of tasks other than treatment. Nor does professional post particularly affect time utilization other than for administrative tasks. This profession has a double objective: care and service more generally and to provide physiotherapy in particular—both equally important. In order to improve the quality of physiotherapy, and at the same time to extend their own specific, theoretical body of knowledge, a number of physiotherapists have reappraised and extended their concept of the profession to include management and research in their everyday work.

    Conclusion: The fact that occupational area exercises such a profound influence on the work of physiotherapists, taken together with the slight influence that professional post has, reveals that the individual physiotherapist must be prepared to play a broadly defined professional role. There seems to be a wealth of skill and expertise available within the profession, which could, however, be more efficiently used if the management and organization of physiotherapy service were better adapted to serve its objectives, and if these were better delineated and communicated.

  • Jeneskog, Torgny
    Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Fysiologi.
    A descending pathway to dynamic fusimotor neurones and its possible relation to a climbing fibre system1974Doktoravhandling, med artikler (Annet vitenskapelig)
  • Disputas: 2017-11-27 13:00 A109, Luleå
    Capaci, Francesca
    Luleå tekniska universitet, Institutionen för ekonomi, teknik och samhälle, Industriell Ekonomi.
    Contributions to the Use of Statistical Methods for Improving Continuous Production2017Licentiatavhandling, med artikler (Annet vitenskapelig)
  • Volgsten, Helena
    et al.
    Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för kvinnors och barns hälsa. Uppsala universitet, Medicinska och farmaceutiska vetenskapsområdet, Medicinska fakulteten, Institutionen för folkhälso- och vårdvetenskap, Vårdvetenskap.
    Schmidt, Lone
    Live birth outcome, spontaneous pregnancy and adoption up to five years after undergoing assisted reproductive technology treatment2017Inngår i: Acta Obstetricia et Gynecologica Scandinavica, ISSN 0001-6349, E-ISSN 1600-0412, Vol. 96, nr 8, 954-959 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    INTRODUCTION: This study is part of a longitudinal cohort undertaken in both women and men to describe live birth outcome after undergoing assisted reproductive technology (ART) treatment in a clinical setting. Another objective was to follow women and men living with children from other alternatives after ART, such as adoption.

    MATERIAL AND METHODS: A total of 439 (80.5%) women and 423 (77.6%) men were included in the baseline cohort (2005-2007). Live birth rate after ART was 24.8% at baseline. Up to 5 years later (2010-2011) the same participants were sent individual postal questionnaires (n = 439).

    RESULTS: Overall, 278 (63.3%) women and 183 (41.7%) men filled in and returned the questionnaire at follow up. The majority of women (91.7%) and men (93.4%) were living with children. A total of 225 (80.9%) women had a live birth at follow up. Of these, almost three of four (71.6%) had a live birth after ART and more than one of four (28.0%) after spontaneous pregnancies or both. Of these, 52 (26.1%) women had a subsequent live birth after successful ART and 26 (32.9%) women after unsuccessful ART. Nineteen (6.8%) women and 13 (7.1%) men had a child after adoption. Almost one of five (19.1%) women had no live birth at follow up.

    CONCLUSION: The majority of women and men were living with children, resulting from a live birth after ART, spontaneous pregnancy and/or adoption up to 5 years later. However, almost one of five had no live birth at follow up.

  • Bergström, Cecilia
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Obstetrik och gynekologi.
    Persson, Margareta
    Umeå universitet, Medicinska fakulteten, Institutionen för omvårdnad.
    Nergard, Kari-Anne
    Mogren, Ingrid
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Obstetrik och gynekologi.
    Prevalence and predictors of persistent pelvic girdle pain 12 years postpartum2017Inngår i: BMC Musculoskeletal Disorders, ISSN 1471-2474, E-ISSN 1471-2474, Vol. 18, 399Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Pelvic girdle pain (PGP) is not always a self-limiting condition. Women with more pronounced persistent PGP (PPGP) report poorer health status compared to women with less pronounced symptoms. The knowledge concerning the long-term consequences of PPGP is limited, thus more knowledge in this area is needed. The overall aim was to study the prevalence and predictors of PPGP 12 years after delivery. Methods: This is a long-term follow-up study based on a previous cohort study that commenced in 2002. New questionnaire data 12 years postpartum were collected in 2014 and early 2015. The questionnaire was distributed to a total of 624 women from the initial cohort. Results: In total, 295 women (47.3%) responded to the questionnaire where 40.3% (n = 119) reported pain to a various degree and 59% (n = 174) reported no pain. Increased duration and/or persistency of pain, self-rated health, sciatica, neck and/or thoracic spinal pain, sick leave the past 12 months, treatment sought, and prescription and/or non-prescription drugs used were all associated with an statistically significant increase in the odds of reporting pain 12 years postpartum. Widespread pain was common and median expectation of improvement score was 5 on an 11-point numeric scale (interquartile range 2-7.50). More than one of five women (21.8%) reporting pain stated that they had been on sick leave the past 12 months and nearly 11% had been granted disability pension due to PPGP. No statistically significant differences were found between respondents and non-respondents regarding most background variables. Conclusions: This study is unique as it is one of few long-term follow-up studies following women with PPGP of more than 11 years. The results show that spontaneous recovery with no recurrences is an unlikely scenario for a subgroup of women with PPGP. Persistency and/or duration of pain symptoms as well as widespread pain appear to be the strongest predictors of poor long-term outcome. Moreover, widespread pain is commonly associated with PPGP and may thus contribute to long-term sick leave and disability pension. A screening tool needs to be developed for the identification of women at risk of developing PPGP to enable early intervention.

  • Boldinova, Elizaveta O.
    et al.
    Stojkovic, Gorazd
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
    Khairullin, Rafil
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik. Kazan Volga Reg Fed Univ, Russia.
    Wanrooij, Sjoerd
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
    Makarova, Alena V.
    Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol2017Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, nr 9, e0184489Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Human PrimPol is a DNA primase/polymerase involved in DNA damage tolerance and prevents nuclear genome instability. PrimPol is also localized to the mitochondria, but its precise function in mitochondrial DNA maintenance has remained elusive. PrimPol works both as a translesion (TLS) polymerase and as the primase that restarts DNA replication after a lesion. However, the observed biochemical activities of PrimPol vary considerably between studies as a result of different reaction conditions used. To reveal the effects of reaction composition on PrimPol DNA polymerase activity, we tested the polymerase activity in the presence of various buffer agents, salt concentrations, pH values and metal cofactors. Additionally, the enzyme stability was analyzed under various conditions. We demonstrate that the reaction buffer with pH 6-6.5, low salt concentrations and 3 mM Mg2+ or 0.3-3 mM Mn2+ cofactor ions supports the highest DNA polymerase activity of human PrimPol in vitro. The DNA polymerase activity of PrimPol was found to be stable after multiple freeze-thaw cycles and prolonged protein incubation on ice. However, rapid heat-inactivation of the enzyme was observed at 37 degrees C. We also for the first time describe the purification of human PrimPol from a human cell line and compare the benefits of this approach to the expression in Escherichia coli and in Saccharomyces cerevisiae cells. Our results show that active PrimPol can be purified from E. coli and human suspension cell line in high quantities and that the activity of the purified enzyme is similar in both expression systems. Conversely, the yield of full-length protein expressed in S. cerevisiae was considerably lower and this system is therefore not recommended for expression of full-length recombinant human PrimPol.

  • Palmqvist, Kristin
    et al.
    Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap.
    Franklin, Oskar
    Nasholm, Torgny
    Symbiosis constraints: Strong mycobiont control limits nutrient response in lichens2017Inngår i: Ecology and Evolution, ISSN 2045-7758, E-ISSN 2045-7758, Vol. 7, nr 18, 7420-7433 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Symbioses such as lichens are potentially threatened by drastic environmental changes. We used the lichen Peltigera aphthosaa symbiosis between a fungus (mycobiont), a green alga (Coccomyxa sp.), and N-2-fixing cyanobacteria (Nostoc sp.)as a model organism to assess the effects of environmental perturbations in nitrogen (N) or phosphorus (P). Growth, carbon (C) and N stable isotopes, CNP concentrations, and specific markers were analyzed in whole thalli and the partners after 4months of daily nutrient additions in the field. Thallus N was 40% higher in N-fertilized thalli, amino acid concentrations were twice as high, while fungal chitin but not ergosterol was lower. Nitrogen also resulted in a thicker algal layer and density, and a higher C-13 abundance in all three partners. Photosynthesis was not affected by either N or P. Thallus growth increased with light dose independent of fertilization regime. We conclude that faster algal growth compared to fungal lead to increased competition for light and CO2 among the Coccomyxa cells, and for C between alga and fungus, resulting in neither photosynthesis nor thallus growth responded to N fertilization. This suggests that the symbiotic lifestyle of lichens may prevent them from utilizing nutrient abundance to increase C assimilation and growth.

  • Gustafsson, Linus
    Försvarshögskolan, Institutionen för säkerhet, strategi och ledarskap (ISSL), CATS (Centrum för Asymmetriska Hot- och Terrorismstudier).
    Terrorismresor2016Annet (Annet vitenskapelig)
  • Disputas: 2017-12-07 22:51 A3024, Luleå
    Samuelsson, Björn
    Luleå tekniska universitet, Institutionen för ekonomi, teknik och samhälle, Industriell Ekonomi.
    Heuristic Mathematical Programming Methods for Lot-sizing, Inventory Control, and Distribution Cost Estimation in the Supply Chain2017Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The supply function has an important role to support the business to create a customer value. Two important parts of this process is to have the warehouses and production sites in the right location and to have the right items stocked at the right level.

     

    This thesis is concerned with those two parts of the supply chain management.

    Three different areas of inventory control are dealt with. In the first part we consider the classical dynamic lot size problem without backlogging. The second part deals with estimation of holding and shortage costs in two-level distribution inventory systems. In the third part of the thesis we consider the localisation problem in a multi-level supply network system where items are consolidated at a warehouse and distributed to customers on routes.

     

    Within the area of inventory control we have evaluated a method earlier suggested by Axsäter (1988), the method is evaluated using a set of test problems and compared other heuristic methods, including the well-known Silver-Meal’s method (Silver and Meal, 1973).  The result shows that the method suggested by Axsäter does perform better than the other methods. In the latest contribution we point to the important differences between Least Period Cost and Silver-Meal when several periods have zero demand. In the area of inventory control we have also studied a two-echelon inventory system where we present methods for estimating the shortage- and stockholding costs in such inventory systems.

     

    The second part subject of the thesis concerns supply network optimization. We present a MIP formulation of the problem and evaluate in detail the approximation of the distribution cost when customers are delivered on multi-stop routes. An improved method for estimating the distribution is presented.

     

    Besides this introductory overview five research papers are included in the thesis. The first and the last paper consider evaluation of dynamic lot sizing heuristics. The second and third paper deals with cost evaluation of a stochastic two-echelon inventory system and the forth paper with evaluation of methods for estimating distribution costs in a supply network.

  • Wennberg, Birgitta
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Centrum för social och affektiv neurovetenskap. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Närsjukvården i centrala Östergötland, Barn- och ungdomspsykiatriska kliniken.
    Janeslätt, Gunnel
    Uppsala University, Sweden; Centre for Clinical Research Dalarna, Falun, Sweden.
    Kjellberg, Anette
    Linköpings universitet, Institutionen för samhälls- och välfärdsstudier, Avdelningen för arbetsterapi. Linköpings universitet, Medicinska fakulteten.
    Gustafsson, Per A
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Centrum för social och affektiv neurovetenskap. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Närsjukvården i centrala Östergötland, Barn- och ungdomspsykiatriska kliniken.
    Effectiveness of time-related interventions in children with ADHD aged 9-15 years: a randomized contolled study2017Inngår i: European Child and Adolescent Psychiatry, ISSN 1018-8827, E-ISSN 1435-165XArtikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Specific problems with time and timing that affect daily routines, homework, school work, and social relations have been recognized in children with ADHD. The primary treatments for children with ADHD do not specifically focus on time-related difficulties. The aim of this randomized controlled study (RCT) was to investigate how multimodal interventions, consisting of training in time-processing ability (TPA) and compensation with time-assistive devices (TAD), affect TPA and daily time management (DTM) in children with ADHD and time difficulties, compared with only educational intervention. Thirty-eight children on stable medication for ADHD in the 9–15-year age range were randomly allocated to an intervention or a control group. The children’s TPA was measured with a structured assessment (KaTid), and the children’s DTM was rated by a parent questionnaire (Time-Parent scale) and by children’s self-reporting (Time-Self-rating). The intervention consisted of time-skill training and compensation with TAD. Data were analysed for differences in TPA and in DTM between the control and intervention groups in the 24-week follow-up. Children in the intervention group increased their TPA significantly (p = 0.019) more compared to the control group. The largest increase was in orientation to time. In addition, the parents in the intervention group rated their children’s DTM as significantly (p = 0.01) improved compared with the parents in the control group. According to the children, their DTM was not significantly changed. In conclusion, a multimodal intervention consisting of time-skill training and TAD improved TPA and DTM in children with ADHD aged 9–15 years.

  • Hoshi Larsson, Martin
    et al.
    Linköpings universitet, Institutionen för ekonomisk och industriell utveckling, Industriell ekonomi. Linköpings universitet, Tekniska fakulteten.
    Ellström, Daniel
    Linköpings universitet, Institutionen för ekonomisk och industriell utveckling, Industriell ekonomi. Linköpings universitet, Tekniska fakulteten.
    Dynamic and static pricing in open-book accounting2017Inngår i: Qualitative Research in Accounting & Management/Emerald, ISSN 1176-6093, E-ISSN 1758-7654, Vol. 14, nr 1, 21-37 s.Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Purpose

    The purpose of this paper is to understand differences between open-book accounting (OBA) using static prices and OBA using dynamic prices. The authors identify how these differences influence various aspects of customer–supplier relationships.

    Design/methodology/approach

    This paper is based on a case study involving a builders’ merchant and a wood manufacturer in the UK. The builders’ merchant under discussion has recently outsourced part of its production to the aforementioned wood manufacturer by using OBA with dynamic prices. For this case study, the authors have conducted interviews with multiple people from both parties in the agreement. Additional illustrative cases are provided through a study of other qualitative papers on OBA.

    Findings

    The authors find evidence supporting that, when dynamic prices are used in OBA, risk (unpredictability) is shifted from the supplier to the customer. Also, the customer frequently focuses on the supplier’s costs, both parties often aim for a long-term relationship and the customer becomes more dependent on the supplier, causing high interdependence. Furthermore, empirical evidence suggests that the customer finds price less important, and the reallocation of activities between the customer and supplier is easier in OBA setups in which dynamic prices are used.

    Originality/value

    This paper provides the first study of how differences between dynamic and static prices in OBA influence the customer–supplier relationship. This paper adds to the developing literature on OBA, in particular, as well as to literature on pricing, in general.

  • Svensson, Josefin
    Uppsala universitet, Universitetsförvaltningen, Kommunikationsavdelningen.
    Universen 5:2017: En tidning för Uppsala universitets medarbetare2017Collection/Antologi (Annet (populærvitenskap, debatt, mm))
    Abstract [sv]

    Innehåll

    Digital arbetsmiljlö i fokus, sid 4

    Återhämtning minskar stressen, sid 5

    Humanistiska teatern invigs, sid 7

    Hon är årets lika villkorspristagare, sid 8

    Nya krav på hantering av personuppgifter, sid 9

    På jobbet: Evolutionsmuseet, sid 10

    På jakt efter nya supraledare, sid 12

    Reportage: Oväntade fynd från vikingatiden, sid 14

    Kompetensutevckling genom intrenationellt utbyte, sid 17

    Satsning på cykling, sid 19

    Profilen: Ashok Swain

  • Bölin, Ingrid
    Umeå universitet, Medicinska fakulteten, Mikrobiologi.
    Temperature-inducible and calcium-regulated proteins encoded by the virulence plasmid of Yersinia1987Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The pathogenic members of the genus Yersinia, Y. pseudotuberculosis, Y. pestis and Y. enterocolitica are transmitted from animals to man and may give rise to disease with a variety of symptoms. These bacteria possess related plasmids necessary for virulence. In this study, gene products encoded by the virulence plasmid have been identified and characterized.

    A temperature-inducible outer membrane protein YOP1, is encoded by the virulence plasmid. YOP1 is expressed by Y. pseudotuberculosis and Y. enterocolitica at 37°C. The genetic locale of trie structural gene for YOPl on the virulence plasmid was determined. A mutant that was unable to express this protein, remained fully virulent, showing that YOP1 is not a virulence determinant.

    Several other proteins encoded by the virulence plasmid are induced at 37°C in a medium lacking Ca2+. These proteins are not expressed at 26°C and expression is repressed by Ca2+-concentrations in excess of 2.5 mM. In Ca2+-deficient medium, the induced proteins can be found extracellu- larly as well as in the outer membrane. However, in the presence of Ca at 37°C they are only found in the outer membrane. The released proteins consist of eight polypeptides as revealed by two-dimensional electro­phoresis. These proteins, Y0P2a and 2b, YOP3, Y0P4a and 4b, the V-antigen and a small uncharacterized polypeptide, are expressed by all three pathogenic Yersinia species, both in vivo and in vitro.

    The Ca2+-controlled expression of the YOP proteins is regulated by genes in the Ca2+ -region, which are conserved in the three species. Mutations in this region repress the expression of the Ca2+-regulated YOPs. The genetic loci identified for five of these proteins revealed that only the structural gene of the Y0P4b protein is part of the Ca2+ -region. The other genes were found at separate locations outside this region. The structural genes for YOP4b, YOP3 and the V-antigen, together with the genes for two additional polypeptides, were localized to a common region conserved on the plasmids of the Yersinia species. The structural genes for Y0P2b (yopH) and Y0P5 (yopE) are located in different positions on the plasmid from Y. enterocolitica, compared to the other two species. This plasmid has Been rearranged so that these genes are located close to one another.

    The DNA sequence of the yopH gene shows that it is a singly transcrip­tional unit. Transcription of this gene is regulated by Ca2+-concentra­tion and by temperature. A mutant strain of Y. pseudo tuberculosis, de­leted for the yopH gene on the virulence plasmid, is avirulent In mice. Virulence is restored by trans-complementation with the cloned yopH gene. The mutant strain is also’ unable to inhibit phagocytosis of macrophages as compared to the wild-type strain. The trans-compleroented strain shows inhibition comparable to that of the wild-type. Therefore, the YOP2b protein is considered to be an essential virulence determinant.

  • Gabrielsson, Linda
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    Gouveia-Figueira, Sandra
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    Häggström, Jenny
    Umeå universitet, Samhällsvetenskapliga fakulteten, Handelshögskolan vid Umeå universitet, Statistik.
    Alhouayek, Mireille
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    Fowler, Christopher J.
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Farmakologi.
    The anti-inflammatory compound palmitoylethanolamide inhibits prostaglandin and hydroxyeicosatetraenoic acid production by a macrophage cell line2017Inngår i: Pharmacology Research & Perspectives, ISSN 2052-1707, Vol. 5, nr 2, UNSP e00300Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The anti-inflammatory agent palmitoylethanolamide (PEA) reduces cyclooxygenase (COX) activity in vivo in a model of inflammatory pain. It is not known whether the compound reduces prostaglandin production in RAW264.7 cells, whether such an action is affected by compounds preventing the breakdown of endogenous PEA, whether other oxylipins are affected, or whether PEA produces direct effects upon the COX-2 enzyme. RAW264.7 cells were treated with lipopolysaccharide and interferon-c to induce COX-2. At the level of mRNA, COX-2 was induced > 1000-fold following 24 h of the treatment. Coincubation with PEA (10 mu mol/L) did not affect the levels of COX-2, but reduced the levels of prostaglandins D-2 and E-2 as well as 11- and 15-hydroxyeicosatetraenoic acid, which can also be synthesised by a COX-2 pathway in macrophages. These effects were retained when hydrolysis of PEA to palmitic acid was blocked. Linoleic acidderived oxylipin levels were not affected by PEA. No direct effects of PEA upon the oxygenation of either arachidonic acid or 2-arachidonoylglycerol by COX-2 were found. It is concluded that in lipopolysaccharide and interferon-c-stimulated RAW264.7 cells, PEA reduces the production of COX-2-derived oxylipins in a manner that is retained when its metabolism to palmitic acid is inhibited.

  • Andersson, Linda
    Umeå universitet, Samhällsvetenskapliga fakulteten, Handelshögskolan vid Umeå universitet, Nationalekonomi.
    Essays on job turnover, productivity and state-local finance2002Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    This thesis consists of four self-contained papers on job turnover, productivity and state- local finance.

    Paper [I] deals with the determinants of the rate of job turnover defined as the change in distribution of employment between and within industries in Swedish manufacturing. The rate of inter-industry job turnover is driven by the dispersion of profit changes among industries. Shifts in international competitiveness among industries seem to play a central role in the explanation of this pattern. The rate of intra-industry job turnover has been higher in industries with many small plants, low profit margins and high import penetration.

    Paper [II] analyzes the impact of openness on total factor productivity (TFP) growth. Using Swedish industry level data the results show that economically integrated industries tend to be more engaged in research and development (R&D) and have more entry and exit activity than other industries. The domestic R&D intensity does not contribute to the TFP growth rate. Instead, the results imply that openness to international markets, which helps facilitate technology spillovers, has a significant impact on the growth rate. There is also some evidence suggesting that producers exiting the market are less productive, implying that such exits will increase the average productivity of the industry concerned.

    The purpose of Paper [III] is to design and implement a test of whether the external effect from tax base sharing among local and regional governments is internalized via the intergovernmental transfer system. The test is based on the observation that if the external effect is internalized, an increase in the income tax rate at one level of government will induce the other level to reduce its income tax rate by the corresponding amount, leaving the effective tax rate unchanged. By using panel data for the Swedish local and regional public sectors, we estimate the reaction function for the local income tax rate. The results imply that an increase in the regional income tax rate induces the municipalities in the region to decrease their income tax rates. In addition, we are able to reject the null hypothesis that the external effect from tax base sharing is internalized.

    Paper [IV] concerns risk-sharing, in terms of how the central government smooths personal income among municipalities via the tax and transfer systems. Using Swedish panel data, the results show that the national tax and transfer systems mitigate an adverse shock to income of one krona so that disposable income falls by 67 öre, on average. However, there are large differences across regions, where the effect on disposable income varies between 32 and 78 öre in the krona.

  • Slagbrand, Tove
    Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för organisk kemi.
    Development and Applications of Molybdenum-Catalyzed Chemoselective Amide Reduction2017Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    This thesis covers the development of catalytic methodologies for the mild and chemoselective hydrosilylation of amides. The first part describes the investigation of the Mo(CO)6-catalyzed reduction of carboxamides. It was found that the reduction could be controlled by tuning the reaction temperature and either amines or aldehydes could be obtained selectively. The system showed an unprecedented chemoselectivity and the amide reduction could take place in the presence of other reducible functional groups such as ketones, aldehydes, and imines. Moreover, the transformation could be performed on a preparative scale and was further employed in the synthesis of Donepezil, a pharmaceutical drug used in the treatment of Alzheimer´s disease.

    The third chapter concerns the development of the Mo(CO)6-mediated hydrosilylation protocol for the reduction of carboxamides containing acidic α-hydrogens. In this case, enamines were formed and a high level of chemoselectivity was observed. Enamines containing sensitive functional groups such as ketones, aldehydes and imines were generated. The enamines were not isolated but used in subsequent catalytic reductive functionalization of amides, which is described in the last part of the thesis (Chapters 4 – 7). The in situ formed enamines were reacted with a wide variety of electrophiles, generating heterocyclic compounds as triazolines, triazoles, 4,5-dihydroisoxazoles and pyrimidinediones. N-sulfonylformamidines as well as thioacrylamides could also be prepared with this approach. The protocols for the synthesis of triazolines, triazoles and N-sulfonylformamidines could additionally be performed on a preparative scale, showing the practicality of the methodology.

  • Yi, Xinlei
    KTH, Skolan för elektro- och systemteknik (EES), Reglerteknik.
    Resource-Constrained Multi-Agent Control Systems: Dynamic Event-triggering, Input Saturation, and Connectivity Preservation2017Licentiatavhandling, monografi (Annet vitenskapelig)
    Abstract [en]

    978-91-7729-579-2A multi-agent system consists of multiple agents cooperating to achieve a common objective through local interactions. An important problem is how to reduce the amount of information exchanged, since agents in practice only have limited energy and communication resources. In this thesis, we propose dynamic event-triggered control strategies to solve consensus and formation problems for multi-agent systems under such resource constraints.

    In the first part, we propose dynamic event-triggered control strategies to solve the average consensus problem for first-order continuous-time multi-agent systems. It is proven that the state of each agent converges exponentially to the average of all agents' initial states under the proposed triggering laws if and only if the underlying undirected graph is connected.In the second part, we study the consensus problem with input saturation over directed graphs. It is shown that the underlying directed graph having a directed spanning tree is a necessary and sufficient condition for achieving consensus. Moreover, in order to reduce the overall need of communication and system updates, we propose an event-triggered control strategy to solve this problem. It is shown that consensus is achieved, again, if and only if the underlying directed graph has a directed spanning tree.In the third part, dynamic event-triggered formation control with connectivity preservation is investigated. Single and double integrator dynamics are considered. All agents are shown to converge to the formation exponentially with connectivity preservation.The effectiveness of the theoretical results in the thesis is verified by several numerical examples.

  • Stanezai, S.
    et al.
    Malmö University.
    Sahlen, E.
    Malmö University.
    El-Schich, Z.
    Fridberg, Marie
    Högskolan Kristianstad, Forskningsmiljön Learning in Science and Mathematics (LISMA). Högskolan Kristianstad, Sektionen för lärande och miljö, Avdelningen för Naturvetenskap.
    Fredriksson, G. N.
    Lund University.
    Anagnostaki, L.
    Skåne University Hospital.
    Tassidis, Helena
    Högskolan Kristianstad, Sektionen för lärande och miljö, Avdelningen för Naturvetenskap. Högskolan Kristianstad, Forskningsmiljön Man & Biosphere Health (MABH).
    Persson, L.
    Lund University.
    Gjorloff Wingren, Anette
    Malmö University.
    Higher intensity of Low Molecular Weight Protein Tyrosine Phosphatase/ ACP-1 in survivors of patients diagnosed with Diffuse Large B Cell Lymphoma (DLBCL) compared to non-survivors2016Inngår i: Austin Biology, nr 2, 1009Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Adult Diffuse Large B Cell Lymphoma (DLBCL) is a heterogeneous form of hematopoietic cancer and difficult to treat. In order to find a better diagnostic indication for the disease, we analyzed Low Molecular Weight Protein Tyrosine Phosphatase (LMWPTP) that in humans is encoded by the ACP1 gene. LMWPTP is an enzyme shown to counteract Protein Tyrosine Kinases (PTK) and was suggested to be a negative growth factor regulator. However, the 18 kDa PTP can also have a positive effect on cell growth and proliferation, indicating a controversial role in the tumorigenic process. LMWPTP exists in different isoforms which are electrophoretically, kinetically and immunologically distinct. We have studied two subgroups of DLBCL consisting of a Germinal Center B cell like (GCB) and a non-Germinal Center B cell like (non-GCB) group. The two subgroups have been defined by gene-expressing profiling and are associated with differential outcome. The expression levels of LMWPTP protein was compared and showed significant differences between the GCB and non- GCB subgroups (p=0.012). Interestingly, when the samples were divided into survivors and non-survivors, and thereafter analyzed for LMWPTP expression, the samples from patients with a higher survival rate showed increased staining intensity, whereas the samples from patients with lower intensity of LMWPTP did not survive the disease (p=0.001). In conclusion, we have shown that DLBCL patients with worse outcome express LMWPTP with a lower intensity, suggesting a tumor suppressor role for this form of the enzyme.

  • Prack, Berit
    Högskolan i Halmstad, Akademin för hälsa och välfärd, Centrum för forskning om välfärd, hälsa och idrott (CVHI), Wigforss-gruppen.
    Aktiv anpassning till förändrade villkor: Fem kvinnors erfarenheter av kroniskt nedsatt funktionsförmåga och återskapande av ett meningsfullt liv2017Licentiatavhandling, monografi (Annet vitenskapelig)
    Abstract [sv]

    Studiens övergripande syfte var att utveckla kunskap om kvinnors

    erfarenheter av att i vuxen ålder drabbas av och leva med kroniskt

    nedsatt fysisk funktionsförmåga. Utöver studiens beskrivande och

    kunskapsgenererande syfte och frågeställningar hade denna även en

    ambition att bidra till utvecklingen av forskningsområdet ”hälsa och

    livsstil”. Studien baserades på aktiva intervjuer med fem kvinnor som

    i vuxen ålder drabbats av nedsatt funktionsförmåga, på grund av kronisk

    sjukdom eller skada. Analysen skedde i flera steg som successivt

    gav en breddad och fördjupad förståelse av kvinnornas berättelser. I

    ett första steg genomfördes en tematisk analys av innehållet i kvinnornas

    berättelser. Den tematiska analysen sammanfattades av hur kvinnorna

    själva genom olika retoriska stilgrepp betonade särskilt viktiga

    delar av sina berättelser. Det andra steget av analysen belyste berättelserna

    utifrån tidigare forskning och den symboliska interaktionismens

    perspektiv. Att studiens resultat relaterades till tidigare forskning och

    ett teoretiskt sammanhang ökade dess överförbarhet ytterligare.

    Studiens resultat låg i linje med tidigare forskning, det bekräftade

    bl.a. tidens betydelse under omställningen till ett liv med nedsatt

    funktionsförmåga, där kontrollen över livet återskapades genom planering

    av tillvaron så att krafterna räckte till det som upplevdes som

    mest angeläget, eller genom att krympa tiden till ett här och nu, och ta

    en dag i sänder och avskärma sig från osäkerheten inför framtiden.

    Det fanns även fynd som gick utöver vad tidigare forskning rapporterat.

    De kvinnor som ingick i studien rekonstruerade ur tidigare erfarenheter

    en ny identitet, med en livsstil i balans mellan omgivningen

    och den faktiska funktionsförmågan. Förhållanden som bidrog till en

    hälsosam livsstil var organiseringen i stödjande nätverk, och engagemang

    i ideella organisationer i vilka kvinnorna kunde uppleva den

    medkänsla som de stundtals sade sig sakna i mötet med vårdgivare

    och myndigheter.

    iii

    De kvinnor som medverkade i studien framstod som mer aktiva och

    kreativa än som var vanligt bland kvinnor med nedsatt funktionsförmåga

    i tidigare studier. Kvinnorna i studien berättade hur de tog initiativ

    till, planerade och genomförde längre livsprojekt, som satsningar

    på studier och eget företagande, vilket inte rapporterats vara vanligt i

    tidigare studier. Några kvinnor visade sin självständighet genom att

    inte villkorslöst acceptera vårdapparatens och myndigheternas beslut,

    utan motsatte sig sådant som motverkade deras strävan att förverkliga

    personliga mål. Kvinnorna stärkte självkänslan och utvecklade alternativa

    livsstilar och identiteter genom att ta vara på tidigare erfarenheter

    och ur dem konstruera nya livsstilar, präglade av relativ hälsa

    och välmående. Det skedde t.ex. genom att utveckla ett idrottsintresse

    i gemenskap med andra inom handikappidrotten, eller genom att vårda

    ett fräscht och ungdomligt utseende och bekräftas genom att medverka

    som modell vid modevisningar.

    Studiens resultat har betydelse för att stärka unga kvinnor, som

    drabbats av nedsatt funktionsförmåga i vuxenålder, men även för yrkesverksamma

    som möter kvinnor i samma situation och deras anhöriga.

  • Dellmuth, Lisa M.
    et al.
    Stockholms universitet, Samhällsvetenskapliga fakulteten, Ekonomisk-historiska institutionen. Stockholms universitet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen.
    Gustafsson, Maria-Therese
    Stockholms universitet, Samhällsvetenskapliga fakulteten, Statsvetenskapliga institutionen.
    Bremberg, Niklas
    Mobjörk, Malin
    Intergovernmental organizations and climate security: advancing the research agenda2017Inngår i: Wiley Interdisciplinary Reviews: Climate Change, ISSN 1757-7780, E-ISSN 1757-7799Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Climate-related security challenges are transnational in character, leading states to increasingly rely on intergovernmental organizations (IGOs) – such as the European Union and the North-Atlantic Treaty Organization – for policy solutions. While climate security issues do typically not fit comfortably within the mandates of existing IGOs, recent decades have seen increasing efforts by IGOs to link climate change and security. This article reviews existing studies on IGOs’ responses to climate security challenges. It draws together research from several bodies of literature spanning political science, international relations, and environmental social science, identifying an emerging field of research revolving around IGOs and climate security. We observe significant advancement in this young field, with scholars extending and enriching our understanding of how and why IGOs address climate security challenges. Yet we still know little about the conditions under which IGOs respond to climate security challenges and when they do so effectively. This article discusses the main gaps in current work and makes some suggestions about how these gaps may be usefully addressed in future research. A better understanding of the conditions under which IGOs respond (effectively) to climate security challenges would contribute to broader debates on climate security, institutional change, and effectiveness in international relations and environmental social science, and may facilitate crafting effective global solutions to society’s most intractable climate security challenges.

  • Olofsson, Gunnar
    Linnéuniversitetet, Fakulteten för samhällsvetenskap (FSV), Institutionen för samhällsstudier (SS).
    En studie av ombuden vid Socialdemokraternas partikongress år 1969: En enkätundersökning av kongressombudens sociala bakgrund, utbildning, yrkesbana och deras organisatoriska förankring i ungdomsförbundet, partiet, fackföreningarna, i riks- och kommunalpolitiken m.m.2017Annet (Annet vitenskapelig)
    Abstract [sv]

    En studie av ombuden vid Socialdemokraternas partikongress år 1969.

    Denna skrift är samling av fyra texter som beskriver en enkätundersökning av kongressombudens sociala bakgrund, utbildning, yrkesbana och deras organisatoriska förankring i ungdomsförbundet, partiet, fackföreningarna, i riks- och kommunalpolitiken m.m.

     Den innehåller följande fyra delar.

     1.  Inledning: Undersökningens tillkomst och bakgrund. 6 si-dor. (oktober 2017)

    2.   P.M. ang. SAP-enkäten 1969. Tekniskt genomförande och råtabeller. 27 sidor. (1974)

    3.   Undersökningens kodschema. 20 sidor (1970)

    4.   Undersökningens frågeformulär. 20 sidor. (1969)

     

  • Disputas: 2017-12-18 09:32 F1031, Luleå
    Anders, Viking
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Industriellt och hållbart byggande.
    Institutional complexity in Swedish built environment regulation: exploring the interface with industrialized house-building2017Doktoravhandling, med artikler (Annet vitenskapelig)
  • Block, Jan
    Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Drift, underhåll och akustik. jan.block@saabgroup.com.
    Part-out Based Spares Provisioning and Management: A Study for Aircraft Retirement2017Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The operation and maintenance phase of a complex technical system may deal with strategicdecisions for asset retirement and end-of-life management. When a fleet of aircraft reachesthe retirement phase, the operation of remaining fleet should still be kept at a defined level ofavailability. Obviously, the provisioning of spares is a key issue to support the maintenanceand operation of the remaining fleet. The best practice within the aviation industry is to re-usethe spares of retired aircraft to support the operational fleet. This is referred to parting-out.The purpose of the research conducted for this thesis has been to develop decision supportmethodologies, models and tools for the management of a sustainable part-out-based sparesprovisioning for an aircraft fleet during its retirement period. The proposed methodology willbe used to support the retirement process of aircraft fleet and enhance the organisation’scapability of making efficient and cost-effective decisions concerning the re-use of spare partsduring the retirement period. To achieve the purpose of this research, literature studies, casestudies, algorithm development and simulations have been conducted. Empirical data havebeen collected through document studies, interviews, and the perusal of archival records fromSaab Support and Services AB. The data analysis performed for this research has been basedon theories and methodologies within reliability analysis, cost modelling, spares forecasting,stock provisioning and decision making, in combination with the best practices implementedby the aviation industry for the end-of-life management and retirement of aircraft.In the present thesis, part-out-based spares provisioning (PBSP) program is proposed to utiliseretired aircraft units effectively as spare parts. The proposed approach is illustrated andverified through a case study performed on the “Saab-105” military aircraft fleet withinSwedish air force fleet. A PBSP programme is proposed, associated management activitiesare described, the key decision criteria are presented, and a functional framework for aneffective PBSP is suggested. The proposed PBSP program provides a foundation for furthermeasures and tasks to be performed within the retirement period, such as terminatingmaintenance contracts, discarding internal maintenance capabilities, reviewing stocks, scalingdown administrative processes (e.g. spares procurement and obsolescence monitoring), etc.An important part of the PBSP programme is the reliability analysis of multiple repairableunits, and this has been investigated, using parametric and non-parametric reliabilityapproaches. The aim is to identify a practical approach for estimation of the future sparedemand at fleet level. Furthermore, a set of computational models and search algorithm havebeen developed for the identification of applicable termination times, of both the parting-outprocess and the maintenance and repair actions performed on the units. This includestermination of the parting-out process (PO), the sending of parted-out units directly to storage(POS), and repair actions performed on the units received at the repair shops owing tocorrective (CM) and preventive (PM) maintenance, as well as the parted-out units that need tobe repaired (POM). The feasible termination alternatives are compared with regard to theirrespective costs and the most cost-effective solutions are identified.The results of the research study show that a PBSP programme can yield large reductions inmaintenance and spares procurement costs, while supporting operation of existing fleet athighest required availability. It also contributes positively to implement a green supply chainduring the retirement phase. The methodology and approaches introduced within the thesiscan be applied in other civil applications, such as energy, mining, process industry andtransportation sectors.

  • Rundegren, Jan
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Kariologi.
    Bacteria-agglutinating glycoproteins in human saliva: an in vitro study with special reference to Streptococcus mutans1982Doktoravhandling, monografi (Annet vitenskapelig)
    Abstract [en]

    The activity and specificity of salivary glycoproteins (agglutinins) aggregating various human indigenous microorganisms were studied in vitro. The agglutinin reacting with a serotype c strain of Streptococcus mutans was isolated and chemically characterized.

    The results can be summarized as follows:

    1. Saliva contains agglutinins against predominant oral microorganisms but also against some of the predominant intestinal bacteria. Agglutinin from one individual was active against microorganisms both from this individual and other individuals.

    2. Saliva-induced agglutination of S. sanguis was inhibited by antisera to IgA, IgG, IgM, and secretory component while S. mutans agglutination was inhibited only by albumin and antisera to IgA. The differences in inhibition indicate the presence of different agglutinins for the two strains.

    3. The agglutinin for an S. mutans serotype c strain was sensitive to a reduction in pH and to treatment with Concanavalin A while an S. mitior agglutinin was not. The tested S. mitior strain, however, adsorbed the S. mutans agglutinin without being agglutinated.

    4. The agglutinin reacting with an S. mutans serotype c strain could be desorbed from the microorganisms in a neutral phosphate buffer. Calcium in the order of 10 million molecules per bacteria was needed to restore agglutination induced by the isolated agglutinin.

    5. The agglutinin in parotid saliva responsible for aggregation of an S. mutans serotype c strain was characterized as a non-immunoglobulin glycoprotein with no blood group activity. The agglutinin was a polymer with a molecular weight exceeding 5 000 kdaltons while the monomeric agglutinin had a molecular weight of 440 kdaltons. The concentration of the agglutinin in parotid saliva was as low as 0.5 per cent of total protein but because 0.1 ug of the agglutinin caused rapid aggregation of as many as 100 million bacteria the agglutinin is considered highly active.

    The results indicate the presence in saliva of different agglutinins with specificity for different bacterial species. High molecular weight glycoproteins seem to play a major role in saliva-induced agglutination of microorganisms and in mechanisms related to clearance and retention of oral microorganisms.

  • Yang, Jie
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC.
    Development of Peptide Binders: Applied to Human CRP, Carbonic Anhydrase (II, IX) and Lysine Demethylase 12017Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    In this thesis, a polypeptide binder concept is illustrated. By conjugation to a set of sixteen polypeptides, a small binding molecule can evolve into a polypeptide binder with increased affinity and selectivity. The concept was applied to 2-oxo-1,2-dihydroquinoline-8-carboxylic acid (DQ) and acetazolamide (AZM) for development of high affinity binders targeting human C-reactive protein (CRP) and human carbonic anhydrase (HCA) II and IX respectively. In addition, peptididic macrocycles were developed as inhibitors of lysine specific demethylase 1 (LSD1).

    CRP is a well-known biomarker of inflammation in humans and binders recognizing it are therefore of large interest as medical diagnostics. Until now, phosphocholine (PCh) and derivatives are the only known small molecule binders for CRP, but they have low μM affinity and bind CRP in a Ca2+ dependent manner. The small molecule DQ was designed as a CRP binder that is structurally unrelated to PCh. Its polypeptide conjugate, 4-C25l22-DQ, was demonstrated as a strong, Ca2+ independent binder for CRP, and had an affinity approximately three orders of magnitude higher than DQ itself.

    HCA IX is a protein that is interesting for diagnosis of cancer. AZM is a small molecule inhibitor of HCAs with a dissociation constant of 38 nM for HCA II and 3 nM for HCA IX. Interestingly, polypeptide conjugate 4-C10L17-AZM displayed stronger binding to both HCA II (KD 4 nM) and HCA IX (KD 90 pM). This result provided evidence that the binder concept can be applied also for small molecules which already have high affinity for their protein receptors.

    LSD1 is an enzyme that regulates the methylation of Lys 4 of histone 3 via a PPI-like interaction and which is of therapeutic interest in certain cancers. Based on the structures of two peptidic ligands bound to LSD1, we sequentially prepared truncated, mono-substituted and macroyclic peptides in order to develop reversible inhibitors of LSD1. Some stapled cyclic peptides bound to LSD1 with 10-fold higher affinity than the corresponding linear parent peptide. Changing the staple into a lactam further improved the binding potency and the best lactams inhibited the enzymatic activity of LSD1 at low μM Ki values.

  • Koch, Günter
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Klinisk fysiologi. Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Pediatrik.
    Lung function and acid-base balance in the newborn infant.1968Doktoravhandling, med artikler (Annet vitenskapelig)
  • Abdurakhmanov, Eldar
    et al.
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Biokemi.
    Solbak, Sara Oie
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Biokemi.
    Danielson, U. Helena
    Uppsala universitet, Teknisk-naturvetenskapliga vetenskapsområdet, Kemiska sektionen, Institutionen för kemi - BMC, Biokemi.
    Biophysical Mode-of-Action and Selectivity Analysis of Allosteric Inhibitors of Hepatitis C Virus (HCV) Polymerase2017Inngår i: Viruses, ISSN 1999-4915, E-ISSN 1999-4915, Vol. 9, nr 6, 151Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Allosteric inhibitors of hepatitis C virus (HCV) non-structural protein 5B (NS5B) polymerase are effective for treatment of genotype 1, although their mode of action and potential to inhibit other isolates and genotypes are not well established. We have used biophysical techniques and a novel biosensor-based real-time polymerase assay to investigate the mode-of-action and selectivity of four inhibitors against enzyme from genotypes 1b (BK and Con1) and 3a. Two thumb inhibitors (lomibuvir and filibuvir) interacted with all three NS5B variants, although the affinities for the 3a enzyme were low. Of the two tested palm inhibitors (dasabuvir and nesbuvir), only dasabuvir interacted with the 1b variant, and nesbuvir interacted with NS5B 3a. Lomibuvir, filibuvir and dasabuvir stabilized the structure of the two 1b variants, but not the 3a enzyme. The thumb compounds interfered with the interaction between the enzyme and RNA and blocked the transition from initiation to elongation. The two allosteric inhibitor types have different inhibition mechanisms. Sequence and structure analysis revealed differences in the binding sites for 1b and 3a variants, explaining the poor effect against genotype 3a NS5B. The indirect mode-of-action needs to be considered when designing allosteric compounds. The current approach provides an efficient strategy for identifying and optimizing allosteric inhibitors targeting HCV genotype 3a.

  • von Ahlefeld Nisser, Désirée
    Högskolan Dalarna, Akademin Utbildning, hälsa och samhälle, Pedagogik.
    Can collaborative consultation, based on communicative theory, promote an inclusive school culture?2017Inngår i: Issues in educational research, ISSN 0313-7155, E-ISSN 1837-6290, Vol. 27, nr 4, 874-891 s.Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    This article contributes to furthering our knowledge of how collaborative consultation, based on communicative theory, can make teachers’ learning from, and with, each other an inclusive process, and thus promote an inclusive school culture. The aim is to study special education professionals’ experiences of, and reflections on, leading collaborative consultations. The data consists of critical reflection (collaborative meta-consultation) in groups of special education professionals (consultants) and one researcher. The focus of the collaborative meta-consultations was on the consultations that the consultants held with groups of teachers. The data has been analysed using qualitative content analysis. Besides identifying some basic conditions, certain strategies and approaches have also been identified to make collaborative consultation an inclusive process. Collaborative consultation can promote an inclusive school culture if the concept of inclusion embraces how professionals collaboratively examine their practice, strategies, and values.

  • Zakrisson, John-Erik
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Öron- näs- och halssjukdomar.
    Experimental studies on the function of the stapedius muscle in man1974Doktoravhandling, med artikler (Annet vitenskapelig)